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Test Code LAB2850 Flow Cytometry PB Malignancy Workup

Important Note

Specimens for PB Malignancy may be collected any time, to be tested the same day or held for testing the following working day. For specimens collected on weekends, holidays, or evening before a holiday, the ordering provider will be responsible for contacting the pathologist on call to arrange workup outside of regular lab hours; otherwise testing will be performed on the next regular working day.

For further information, contact Hematopathology (Cell Markers) at 206-987-2560 or Pathology 206-987-2103.

Clinical System Name

Blood Flow Malignancy Flow Workup Request

Synonyms

Peripheral Blood Malignancy Workup

Blood Malignancy Flow Workup Request
Flow Cytometry Immunophenotyping (Leukemia)
Immunophenotyping
Malignancy, Peripheral Blood
Peripheral Blood Malignancy Workup by Flow Cytometry
Routine Immunophenotyping

Blood Malignancy Flow Workup

Bld Malig

Sample Requirements

Specimen:  Whole Blood

Container(s):  Lavender/EDTA; Dark Green/Heparin also acceptable

Preferred Vol:  5 mL; volume dependent on blast count 

Minimum Vol:  dependent on blast count; consult lab

Note:  Specimens for PB Malignancy may be collected any time, to be tested the same day or held for testing the following working day. This test is for peripheral blood workups only - for bone marrow, see Bone Marrow listings.

Processing Instructions

Reject due to:  n/a - send to lab

Spin:  N

Aliquot:  N

Temp:  RT

Storage location: Days: M - F until 1700, transport specimen and labels to the Cell Markers Lab (station #181); call before tubing specimen to make sure there is someone available to receive and perform testing.

After hours: Store sample in Cell Markers RT box in CPA. 

Off-site collection: Keep specimen at room temperature and transport to Children's Lab. Please contact Laboratory Client Services at (206) 987-2617 with method of transport and shipping number.

Stability

Temperature Time
Room temp 30 hrs in EDTA or 48 hrs in heparin
Refrigerated N
Frozen N

 

Availability

STAT Performed TAT
Y - with pathologist approval

Monday - Friday 0800-1630; Other times as arranged through pathologist on-call.  Weekend, holiday, pm before holiday, provider must contact pathologist on-call. SEE ORDERING NOTES ABOVE.

2-3 days

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Cell Markers

Phone Number: 206-987-2560

CPT Codes

Contact Laboratory Client Services at (206)-987-2617

Methodology

Method:  Flow Cytometry

Analytical Volume:  dependent on blast count; consult lab

Limitations:  None specified

Reference Range

None specified
 

Description

Flow Cytometry PB Malignancy Workup is used in determining the lineage of hematopoietic precursors and in classifying acute leukemias. This workup may be performed on peripheral blood if there are sufficient blasts for evaluation; requests will be evaluated by pathologist.