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Test Code LAB2870 C5 Nephritic Factor

Important Note

Specimen must be received by the Main Lab within 30 minutes of collection.

 

CPA: Separate serum from cells and freeze within ONE hour of collection.

Additional Codes

C5 Neph

Clinical System Name

C5 Nephritic Factor

Sample Requirements

Specimen: Whole Blood

Container(s): Red

Preferred Vol: 6.0 mL

Minimum Vol: 4.0 mL

 

Note: Draw blood in a Red top tube. Gold/SST (serum separator tubes) are not acceptable.

Processing Instructions

Reject due to: Gold/SST

Spin: Y

Aliquot: Y

Temp: -70 C

Storage Location: CRITICAL FROZEN. Spin blood roughly 30 minutes after collection, transfer 3.0 mL serum (Min. 1.0 mL) to a plastic aliquot tube affixed with large Epic label. Deliver specimen to the CPA -70 freezer, Send Outs rack. Serum should be frozen within ONE hour of blood draw.

 

Off-Site Collection: Spin blood roughly 30 minutes after collection, transfer 3.0 mL serum (Min. 1.0 mL) to a plastic tube affixed with large Epic label. Freeze at -70 C. Serum should be frozen within ONE hour of blood draw. Transport frozen.

Stability

Specimen Type Temperature Time
Serum Room temp Unacceptable
  Refrigerated Unacceptable
  Frozen Y

 

Availability

STAT Performed TAT
N Daily 4 w

 

Performing Laboratory

The University of Iowa

Molecular Otolaryngology & Renal Research Laboratories (MORL)

285 Newton Rd, 5270 CBRB

Iowa City, IA 52242-1078

 

Phone: (319)-335-6623

 

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

86235, 86161

Methodology

Method: C3 Convertase Stabilizing Assay with Properdin (C3CSAP) measures the ability of C3Nefs to stabilize C3 convertase built with properdin on sheep erythrocytes. Stabilizing activities are reported as a function of hemolysis at 40 minutes.

Analytical Volume: 1.0 mL Serum

Limitations:

Reference Ranges

Interpretive report provided.

Send Out Instructions

Reference Test Name: C5 Nephritic Factor
Reference Lab Test Code: C5Nef (C3CSAP)
Instructions Ship Monday through Thursday via FedEx Priority Overnight. Saturday deliveries are not accepted.