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Test Code LAB2899 CD107a Mobilization

Important Note

Collect Monday through Wednesday 1000 - 1400 ONLY. No regional site collections.

Additional Codes

CD107a Mob

Clinical System Name

CD107a Mobilization

Synonyms

HLH

NK cell degranulation

CD107a NK cell

Sample Requirements

Specimen: Whole Blood

Container(s): Dark Green/Sodium Heparin

Preferred Vol: See Table Below

Minimum Vol: Call Send Outs at (206) 987-2563

 

Note: Samples must be drawn Monday through Wednesday, 1000 - 1400 ONLY. Samples drawn outside of these times will be rejected. If drawn outside of the times, contact the Send Outs department at ext. 7-2563 for further clarification. Send sample(s) to Main Lab immediately after collection. Specimens are stable for only 24 hours at room temperature.

 

ALC

(K/mcL)

ALC

(cells/mcL

or cells/mm3)

 mL of Sodium Heparin blood required
>1.00 >1000 5.0
1.00 1000 5.0
0.90 900 5.5
0.80 800 6.0
0.70 700 6.5
0.60 600 7.5
0.50 500 9.0
0.40 400 11.0
0.30 300 15.0
0.20* 200* 25.0*
0.10* 100* 45.0*
<0.10* <100* 50.0*

IMPORTANT NOTE: *For Absolute Lymphocyte Counts (ALC) below 200 cells/mcL, consider waiting until count increases.

Processing Instructions

Reject due to: Frozen, clotted, hemolyzed or centrifuged specimens. Specimen collected in an unacceptable anticoagulant.

Spin: N

Aliquot: N

Temp: RT

Storage Location: Affix a large Epic label to the sample(s), place in the room temperature Send Outs rack, and notify the Send Outs department.

 

Off-site Collection: No regional site collections.

Stability

Specimen Type Temperature Time
  Room temp 24 h
  Refrigerated Unacceptable
  Frozen Unacceptable

 

Availability

STAT Performed TAT
N M - Th 5 d

Performing Laboratory

Cincinnati Children’s Hospital

Diagnostic Immunology Laboratory

DIL - Rm R2328

3333 Burnet Avenue

Cincinnati, OH 45229-3039

 

Phone: (513) 636-4685

Department

Department: Send Outs

Phone: (206) 987-2563

CPT Codes

88184, 88185 (x3), 88187, 86352

Methodology

Method: Flow Cytometry

Analytical Volume: See "Sample Requirements" section for more information.

Limitations: This is a 2-day test and cannot be accepted on Friday or the day before a holiday.

Reference Range

Interpretive report is provided.

Send Out Instructions

Reference Test Name: CD107a Mobilization (NK Cell Degranulation)
Reference Lab Test Code: 2905400
Instructions: Ship ambient, Monday through Wednesday, via FedEx Priority Overnight. Saturday deliveries are not accepted.