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Test Code LAB2900 Hemoglobin Electrophoresis

Important Note

Hemoglobin electrophoresis alone is not adequate for diagnosing thalassemia. Electrophoresis is limited to identification without fractionation, quantification or interpretation. Consider ordering “Hemoglobin Disorder Thalassemia Reflexive Panel” which includes hemoglobin electrophoresis, isoelectric focusing, BCB prep, zinc protoporphyrin, and includes an interpretation.

Additional Codes

HB Elect

Clinical System Name

Hemoglobin Electrophoresis

Synonyms

HB ELP; Hemoglobinopathy; red cell; Sickle Cell Trait; Sickle Cell-Routine; Sickle Dex-Routine

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA or Lavender/EDTA Microtainer

Preferred Vol: 3.0 mL

Minimum Vol: 1.5 mL

 

Note: Macrotainer samples preferred.

Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: 2 - 4 C

Storage location: Refrigerate whole blood in CPA refrigerator Send Outs rack.

If a variant Hemoglobin is detected, confirmatory citrate agar electrophoresis will be performed as required for diagnosis.

 

Off-site collection:

Stability

Specimen Type Temperature Time
Whole Blood Room temp 2 d
  Refrigerated 7 d
  Frozen n/a

 

Availability

STAT Performed TAT
N 3 - 4 times/w 5 d

 

Performing Laboratory

Harborview Medical Center

325 9th Ave, Room GWH47

Seattle, WA 98104

 

Phone Number: (206) 744-3451

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

83020

Methodology

Method: Isolectric Focusing Electrophoresis (IEF)

Analytical Volume: 1.5 mL Whole Blood

Limitations:

Reference Range

See report.

Send Out Instructions

Reference Test Name: Hemoglobin Electrophoresis (Isoelectric Focusing)
Reference Test Number: HBELEC
Instructions: Send out Monday through Friday with the UW courier.