Home
HelpTest Code LAB2902 CD34 Peripheral Stem Cells
Additional Codes
LAB3696 CD34 Peripheral Stem Cells (SCCA)
Clinical System Name
CD34 Peripheral Stem Cells
Synonyms
CD 34
Stem Cell Assay
CD34 Peripheral Stem Cells
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender/EDTA
Preferred Vol: 5 mL
Minimum Vol: Based on patient's WBC count; consult lab
Note: For clinically useful result turnaround, sample should be drawn before NOON Monday - Friday.
Processing Instructions
Reject due to: clotted specimen
Spin: N
Aliquot: N
Temp: RT (Please see information below about Fred Hutch samples and refrigerator storage)
Note: If WBC is not ordered on same draw, have the Core Lab run specimen through hematology analyzer and attach copy of results with specimen to Cell Markers (not required for specimens to FHCC).
| STORAGE LOCATION/SPECIMEN TRANSPORT | |
|---|---|
| M-F | Days: Notify Cell Markers Lab when specimen arrives. Transport specimen with copy of WBC to the Cell Markers Lab (Station #181). Eves/Nights: Store specimen, copy of results, and labels in Cell Markers RT box in CPA. |
| Saturday |
Saturday early morning (MIDNIGHT to 8 AM): Store specimen, copy of results, and labels in Cell Markers RT box in CPA. Samples outside of these hours on weekends/holidays must be reviewed with the Lab Medicine on call prior to collection. Samples can be sent to Fred Hutch Cellular Therapy Lab (206) 606-1200, but the TAT is >24 hrs and the delay will likely impact clinical significance. Note for approved send out: If sample is received outside of FHCC hours (9 AM to 3 PM) refrigerate sample and notify next morning shift to send at 8 AM. 1. Cancel CD34 order and reorder as SCCA CD34. 2. Use SCCA CD34 requisition (Green Form) next to Room Temp Sendouts rack. 3. Log in to Delivery Express and select "CPA-CD34 Weekend" from location dropdown menu. 4. Make a copy of the SCCA requisition and the Delivery Express shipping label. Staple together and place labels in Sendouts box for documentation. 5. Samples are sent at 8 am on Sundays and Holidays. Please leave a voicemail for the FH Cellular Therapy Lab at 206-606-1200 that a package has been sent. |
| Sundays & Holiday |
Samples must be reviewed with the Lab Medicine on call prior to collection. Samples can be sent to FH Cellular Therapy Lab (206) 606-1200, but the TAT is >24 hrs and the delay will likely impact clinical significance. Review to directions above if send out testing is approved. |
Off-site collection: Not available
Stability
| Temperature | Time |
|---|---|
| Room temp | Stability limited - draw in AM before NOON and process immediately |
| Refrigerated | N for In-house tested samples (Y for approved FHCC samples) Please see information under Processing Instructions. |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| always STAT | Daily. M-Sat test is run at Children's Lab. Sun and holidays, approved testing is run at Fred Hutchinson (formerly SCCA) Cellular Therapy Lab. CPA, please cancel CD34 order and order SCCA CD34. | same day |
Performing Laboratory
Seattle Children's Laboratory (M-Sat)
Fred Hutch Cellular Therapy Lab (Sun or holiday, with approval)
1100 Eastlake Ave E
Rm E1-1415
Seattle, WA 98109
Phone Number: (206) 606-1200
Department
Department: Cell Markers (M-Sa)
Phone Number: 206-987-2560
Reference Lab (Sun and holidays):
Fred Hutchinson Cellular Therapy Lab
1100 Eastlake Ave E
Rm E1-1415
Seattle, WA 98109
(206) 606-1200
CPT Codes
86367
Methodology
Method: Flow Cytometry (ISHAGE protocol)
Analytical Volume: dependent on patient white count; consult lab
Limitations:
Reference Range
There are no reference ranges established for mobilized CD34 levels.
Description
The purpose of this assay is to identify and quantify hematopoietic stem cells. The methodology used in this assay is based on the ISHAGE guidelines for dual platform CD34 flow cytometry determinations.