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HelpTest Code LAB2916 Complement, Total, Serum
Reporting Name
Complement, Total, SSCH Clinical System Name: CH50 Total Hemolytic Complement
Useful For
Detection of individuals with an ongoing immune process
First-tier screening test for congenital complement deficiencies
Specimen Required
Patient Preparation: Fasting preferred but not required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Immediately after specimen collection, place the tube on wet ice and allow specimen to clot.
2. Centrifuge at 4° C and aliquot serum into 5 mL plastic vial.
3. Within 30 minutes of centrifugation, freeze specimen. Specimen must be placed on dry ice if not frozen immediately.
NOTE: If a refrigerated centrifuge is not available, it is acceptable to use a room temperature centrifuge, provided the specimen is kept on ice before centrifugation, and immediately afterward, the serum is aliquoted and frozen.
Collect 2.0 mL whole blood in a Red Top.
Reject due to: SST, specimens stored refrigerated or ambient.
Note: Allow specimen to clot 15-30 min (on ice). Centrifuge and separate serum from cells within TWO hours of collection. Transfer 1.0 mL serum (Min. 0.5 mL) to an aliquot tube affixed with large Epic label. Freeze (immediately) at -20 C.
Specimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen | 28 days |
Day(s) Performed
Monday through Friday
TAT: 3 - 4 days
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Automated Liposome Lysis Assay
Method Description
An automated method is performed using liposomes as the target for the serum complement system. Dinitrophenyl (DNP)-labeled liposomes are sensitized with antibody to DNP. Serum complement causes lysis and release of entrapped glucose-6-phosphate dehydrogenase. Glucose-6-phosphate dehydrogenase reacts with glucose-6-phosphate and nicotinamide adenine dinucleotide (NAD[+]). NAD(+) is then reduced to NADH, and the conversion is measured at 340 nm. The assay correlates with the total complement assay based on sheep red blood cell lysis, has lower variability, and is simpler to perform. (Package insert: Fujifilm Autokit CH50. Fujifilm Wako Pure Chemical Corporation; 04/2018; Yamamoto S, Kubotsu K, Kida M, et al. Automated homogeneous liposome-based assay system for total complement activity. Clin Chem. 1995;41[4]:586-590)
Reference Values
30-75 U/mL
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86162
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| COM | Complement, Total, S | 4532-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| COM | Complement, Total, S | 4532-8 |
Report Available
1 to 2 daysReject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |