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Test Code LAB293 CBC + DIFF

Important Note

Manual differentials are automatically performed when specific criteria are met in the auto differential; manual differentials cannot be ordered.

 

Add ons for differentials can only be performed within 48 hours of the CBC collection.

Clinical System Name

Complete Blood Count with Differential

Synonyms

CBC DIFF

LAB293

Sample Requirements

Specimen: Whole Blood

Container(s):  Lavender/EDTA

Preferred Vol:  2.0-4.0 mL

Minimum Vol:  0.5 mL Microtainer; 1.0 mL Macrotainer

 

Notes: A Lavender top/EDTA Macrotainer must contain at least 1.0 mL.

Processing Instructions

Reject due to:  Insufficient Quantity, Wrong Sample Collection, Clotted Sample, Age of specimen

Spin: N

Aliquot: N

Temp: RT

Storage location:  Core 5 Fridge

 

Off-site collection: Store whole blood at room temperature and transport to Children's Lab immediately. Samples > 24 hours old may be rejected.

Stability

Specimen Type Temperature Time
Whole blood RT

≤ 24 h

Whole blood 2-8 C

≤ 48 h

  -20 C or -70 C

N

Availability

STAT Performed TAT
Y 24/7 1 h

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Hematology

Phone Number: 206-987-2617 (Client Services)

CPT Codes

85025

Methodology

Method:  Sysmex XN-3000, XN-1000, & XN-350: RF/DC detection method, Flow cytometry with semiconductive laser, photometrically, SLS-hemoglobin method, hydrodynamic focusing

Analytical Volume:  0.25 mL  whole blood

Reference Range

Please refer to "CBC No Diff"  for Red cell indices reference ranges.

WBC <0.2: No differential.  Differential and RBC morphology are automatically resulted with "No Data" and final verified.

 

Test

Age Range (Male & Female)

    %

   /CMM

Polys

0 - 1 Day

33 - 70   

3000 - 12000

Polys

1 Day - 1 Week

15 - 50

2000 - 6000

Polys

1 Week - 1 Month

15 - 45

1500 - 5000

Polys

1 Month - 1 Year

15 - 45

1500 - 5000

Polys

1 - 4 Years

15 - 70

1500 - 7500

Polys

4 - 10 Years

25 - 70

1800 - 7000

Polys

10 - 14 Years

30 - 70

1500 - 7000

Polys

14 Years - Adult

40 - 75

1500 - 7000

Bands

0 - 24 Hrs

0 - 9

0 - 1500

Bands

1 Day - 1 Week

0 - 9

0 - 1200

Bands

1 Week - 1 Month

0 - 9

0 - 500

Bands

1 Month - 1 Year

0 - 9

0 - 350

Bands

1 - 4 Years

0 - 9

0 - 270

Bands

4 - 10 Years

0 - 9

0 - 240

Bands

10 Years - Adult

0 - 9

0 - 200

Lymphocytes

0 - 1 Day

10 - 35

2000 - 11000

Lymphocytes

1 Day - 1 Week

15 - 70

2000 - 7000

Lymphocytes

1 Week - 4 Years

30 - 70

3000 - 7000

Lymphocytes

4 - 6 Years

20 - 70

1500 - 8500

Lymphocytes

6 - 10 Years

20 - 50

1500 - 5000

Lymphocytes

10 Years - Adult

20 - 40

1100 - 4500

Monocytes

0 Days - 1 Month

0 - 10

0 - 1100

Monocytes

1 Month - Adult

0 - 10

0 - 900

Eosinophils

0 - 24 Hrs

0 - 4

0 - 400

Eosinophils

1 Day - 1 Week

0 - 4

0 - 500

Eosinophils

1 Week - 1 Year

0 - 4

0 - 300

Eosinophils

1 - 10 Years

0 - 4

0 - 250

Eosinophils

10 Years - Adult

0 - 4

0 - 200

Basophils

0 - 1 Week

0 - 1

0 - 100

Basophils

1 Week - 4 Years

0 - 1

0 - 50

Basophils

4 Years - Adult

0 - 1

0 - 40

Immature Granulocytes 0 - Adult 0 - 0.7 0 - 50

Description

Examination of the numerical complete blood count is useful in diagnosis of such disease states as anemias, leukemias, allergic reactions, viral, bacterial, and parasitic infections.

 

This test includes WBC, RBC, Hgb, Hct, MCV, MCH. MCHC, RDW, Plt, MPV, red cell morphology, white cell differential, and Absolute Neutrophil Count (ANC).

Critical Values

Absolute Neutrophil Count < 500/CMM