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HelpTest Code LAB293 CBC + DIFF
Clinical System Name
Complete Blood Count with Differential
Description
Examination of the numerical complete blood count is useful in diagnosis of such disease states as anemias, leukemias, allergic reactions, viral, bacterial, and parasitic infections.
This test includes WBC, RBC, Hgb, Hct, MCV, MCH. MCHC, RDW, Plt, MPV, red cell morphology, white cell differential, and Absolute Neutrophil Count (ANC).
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender/EDTA
Preferred Vol: 2.0-4.0 mL
Minimum Vol: 0.5 mL Microtainer; 1.0 mL Macrotainer
Notes: A Lavender top/EDTA Macrotainer must contain at least 1.0 mL.
Processing Instructions
Reject due to: Insufficient Quantity, Wrong Sample Collection, Clotted Sample, Age of specimen
Spin: N
Aliquot: N
Temp: RT
Storage location: Core 5 Fridge
Off-site collection: Store whole blood at room temperature and transport to Children's Lab immediately. Samples > 24 hours old may be rejected.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | RT |
≤ 24 h |
| Whole blood | 2-8 C |
≤ 48 h |
| -20 C or -70 C |
N |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Core Hematology
Phone Number: 206-987-2617 (Client Services)
Synonyms
CBC DIFF
LAB293
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y | 24/7 | 1 h |
Methodology
Method: Sysmex XN-3000, XN-1000, & XN-350: RF/DC detection method, Flow cytometry with semiconductive laser, photometrically, SLS-hemoglobin method, hydrodynamic focusing
Analytical Volume: 0.25 mL whole blood
Reference Range
As of 11/18/2025, Polymorphonuclear (Polys) and Band cell populations are no longer reported as distinct cell types. The Neutrophil Count % and the Absolute Neutrophil Count include both polymorphonuclear and band cells.
Please see CBC for Red cell indices reference ranges.
Note: No automated differential is reported when WBC <200/CMM. Differential and RBC morphology are automatically resulted with "No Data."
| Test | Age Range (Male & Female) | % | /CMM |
|---|---|---|---|
| Neutrophil Count % | 0 - 1 Day | 33 - 70 | 3000 - 12000 |
| Neutrophil Count % | 1 Day - 1 Week | 15 - 50 | 2000 - 6000 |
| Neutrophil Count % | 1 Week - 1 Month | 15 - 45 | 1500 - 5000 |
| Neutrophil Count % | 1 Month - 1 Year | 15 - 45 | 1500 - 5000 |
| Neutrophil Count % | 1 - 4 Years | 15 - 70 | 1500 - 7500 |
| Neutrophil Count % | 4 - 10 Years | 25 - 70 | 1800 - 7000 |
| Neutrophil Count % | 10 - 14 Years | 30 - 70 | 1500 - 7000 |
| Neutrophil Count % | 14 Years - Adult | 40 - 75 | 1500 - 7000 |
| Lymphocyte Count % | 0 - 1 Day | 10 - 35 | 2000 - 11000 |
| Lymphocyte Count % | 1 Day - 1 Week | 15 - 70 | 2000 - 7000 |
| Lymphocyte Count % | 1 Week - 4 Years | 30 - 70 | 3000 - 7000 |
| Lymphocyte Count % | 4 - 6 Years | 20 - 70 | 1500 - 8500 |
| Lymphocyte Count % | 6 - 10 Years | 20 - 50 | 1500 - 5000 |
| Lymphocyte Count % | 10 Years - Adult | 20 - 40 | 1100 - 4500 |
| Monocytes Count % | 0 Days - 1 Month | 0 - 10 | 0 - 1100 |
| Monocytes Count % | 1 Month - Adult | 0 - 10 | 0 - 900 |
| Eosinophil Count % | 0 - 24 Hrs | 0 - 4 | 0 - 400 |
| Eosinophil Count % | 1 Day - 1 Week | 0 - 4 | 0 - 500 |
| Eosinophil Count % | 1 Week - 1 Year | 0 - 4 | 0 - 300 |
| Eosinophil Count % | 1 - 10 Years | 0 - 4 | 0 - 250 |
| Eosinophil Count % | 10 Years - Adult | 0 - 4 | 0 - 200 |
| Basophils Count % | 0 - 1 Week | 0 - 1 | 0 - 100 |
| Basophils Count % | 1 Week - 4 Years | 0 - 1 | 0 - 50 |
| Basophils Count % | 4 Years - Adult | 0 - 1 | 0 - 40 |
| Immature Granulocytes % | 0 - Adult | 0 - 0.7 | 0 - 50 |
CPT Codes
85025
Critical Values
| Absolute Neutrophil Count | < 500/CMM |