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Test Code LAB2952 Cortrosyn Stimulation 0+30 Minute Cortisols

Important Note

ACTH and Leuprolide stimulation tests are only scheduled and administered at Sand Point Endocrine Clinic or SCH inpatients.

Clinical System Name

Cortrosyn Stimulation 0+30 Min

Synonyms

CORTROS 30

Sample Requirements

Specimen: Whole blood

Container(s): Gold SST, Dark Green/Sodium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold Microtainer, Red

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL

Note: Draw samples at 0 and 30 minutes post Cortrosyn (ACTH). Note collection times on requisitions and samples. 

Note: Capillary collection is unacceptable for cortisol testing. 

Processing Instructions

Reject due to: 

Spin: Y

Aliquot: Y

Temp: 2-8 C

Storage location: CPA refrigerator, FrigD rack. Serum/plasma should be removed from separator for storage.

 

Off-site collection: Draw samples at 0 and 30 minutes post Cortrosyn (ACTH). Note collection times on requisitions and samples. Spin blood.  Transfer serum/plasma to plastic aliquot container. Refrigerate.

Stability

Specimen Type Temperature Time
gel separator with serum/plasma Refrigerated 8 h
separated serum/plasma Room temp 2 h
  Refrigerated 14 d
  Frozen -20 C 30 d

 

Availability

STAT Performed TAT
N M-Sa, day shift 1-3 d

 

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Chemistry

Location: Chem West

Phone Number:  (206) 987-2617

 

 

 

CPT Codes

82533

Methodology

Method: Chemiluminescent microparticle immunoassay (CMIA) on the Alinity i

Analytical Volume: 0.150 mL serum

Limitations: 

Reference Range

Cortisol  mcg/dL 
Baseline (8-10 AM)  3.7 -19.4
Post ACTH 30 min > 13.2