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Test Code LAB296 Reticulocyte

Clinical System Name

Reticulocyte Count

Synonyms

Retic

LAB296

Description

The circulating reticulocyte count is the most convenient method for evaluating effective marrow production of erythrocytes.

Sample Requirements

Specimen: Whole Blood

Container(s):  Lavender/EDTA

Preferred Vol:  1.0-3.0 mL

Minimum Vol:  0.5 mL Microtainer; 1.0 mL Macrotainer

 

Notes: A Lavender top/EDTA Macrotainer must contain at least 1.0 mL.

Processing Instructions

Reject due to:  Insufficient Quantity, Wrong Sample Collection, Clotted Sample, Age of specimen

Spin: N

Aliquot: N

Temp: RT

Storage location:  Core 5 Fridge

 

Off-site collection: Store whole blood at room temperature and transport to Children's Lab immediately. Samples > 24 hours old may be rejected.

Stability

Specimen Type Temperature Time
Whole blood RT

≤ 24 h
Whole blood 2-8 C

≤ 48 h
  -20 C or -70 C

N

Availability

STAT Performed TAT
Y 24/7 1 h

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Hematology

Phone Number: 206-987-2617 (Client Services)

Methodology

Method:  Sysmex XN-3000 & XN-1000: Flow cytometry with semiconductive laser

Analytical Volume:  0.25 mL whole blood

Reference Range

Test

Age

Range
Reticulocyte

0 - 1 Day

 1.80 - 4.60%
Reticulocyte

1 Day - 1 Week

0.10 - 0.90%
Reticulocyte

1- 4 Weeks

0.30 - 0.90%
Reticulocyte

4 - 6 Weeks

0.30  - 1.70%
Reticulocyte

6 - 8 Weeks

0.80 - 2.20%
Reticulocyte

8 - 10 Weeks

0.50 - 1.90%
Reticulocyte

10 - 12 Weeks

0.40 - 1.00%
Reticulocyte

12 Weeks - Adult

0.50 - 1.50%
Ret-He* 0 - Adult 28 pg - 38 pg
Raw Retic ABS   0 - Adult 0.018 – 0.100 M/mm3　

*Reticulocyte hemoglobin equivalent (Ret-He) is a parameter dependent on the hemoglobin content of the reticulocytes. Ret-He is usually decreased in patients with iron deficiency anemia (IDA). IDA could be diagnosed with a sensitivity of 93% and specificity of 83% when the Ret-He is low.

CPT Codes

85045