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HelpTest Code LAB2962 CRH Stimulation Cortisol Levels
Clinical System Name
CRH Stimulation Cortisol Levels
Sample Requirements
Specimen: Whole blood
Container(s): Gold SST, Dark Green/Sodium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold Microtainer, Red
Preferred Vol: 1.0 mL
Minimum Vol: 0.5 mL
Note: Draw samples at 0, 30, 60, 90 minutes post CRH. Note collection times on requisitions and samples.
Note: Capillary collection is unacceptable for cortisol testing.
Processing Instructions
Reject due to:
Spin: Y
Aliquot: Y
Temp: 2-8 C
Storage location: CPA refrigerator, FrigD rack. Serum/plasma should be removed from separator for storage.
Off-site collection: Draw samples at 0, 30, 60, 90 minutes post CRH. Note collection times on requisitions and samples. Spin blood. Transfer serum/plasma to plastic aliquot container. Refrigerate.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| gel separator with serum/plasma | Refrigerated | 8 h |
| separated serum/plasma | Room temp | 2 h |
| Refrigerated | 14 d | |
| Frozen -20 C | 30 d |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M-F, day shift | 1-4 d |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Chemistry
Location: Chem West
Phone Number: (206) 987-2617
CPT Codes
82533
Methodology
Method: Chemiluminescent microparticle immunoassay (CMIA) on the Alinity i
Analytical Volume: 0.150 mL
Limitations:
Reference Range
| CORTISOL | mcg/dL |
| Baseline (8-10 AM) | 3.7 -19.4 |
| Post CRH | 2-3 times baseline |