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Test Code LAB2967 Tobramycin - Cystic Fibrosis Patients

Important Note

No add-ons after 8 hours of collection if specimen is still on gel separator.

Clinical System Name

Cystic Fibrosis Tobramycin Level

Synonyms

CF TOBRA

LAB2967

Sample Requirements

Specimen:  Whole Blood

Container(s): Lt. Green/Mint Top Lithium Heparin, Lt. Green/Lithium Heparin Microtainer, Gold SST, Gold Microtainer, Dark Green/Sodium Heparin, Red, Lavender/EDTA, Lavender/EDTA Microtainer

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL

 

Note: Every 24 hour dosing: Infuse IV over 30 minutes. Upon admission, initial levels should be drawn following the 2nd dose. Levels should be drawn at 2 and 6 hours after the end of the IV infusion. Document the date and the time of infusion and collections. Following a dose adjustment during a single admission, repeat 2 and 6 hour levels should follow the 1st dose of the new dose regimen.

Processing Instructions

Reject due to:  Insufficient quantity, Age of specimen

Spin: Y

Aliquot: Y

Storage location:  Core Chemistry Refrigerator

 

Off-site collection:  Every 24 hour dosing: Infuse IV over 30 minutes. Upon admission, initial levels should be drawn following the 2nd dose.  Levels should be drawn at 2 and 6 hours after the end of the IV infusion. Document the date and the time of infusion and collections. Following a dose adjustment during a single admission, repeat 2 and 6 hour levels should follow the 1st dose of the new dose regimen. Specimen should be centrifuged within 1 hour of collection. Spin blood and transfer serum or plasma to plastic tube and refrigerate or freeze.

Stability

Specimen Type Temperature Time
Serum or Plasma RT

≤ 2 h
Serum or Plasma 2-4 C

≤ 7 d
Serum or Plasma -20 C or -70 C

≤ 14 d

Availability

STAT Performed TAT
Y 24/7 1 h

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Chemistry

Phone Number: 206-987-2617 (Client Services)

CPT Codes

80200

Methodology

Method:  Two Point Rate method by Vitros 4600

Analytical Volume: 0.06 mL + 0.03 mL dead space

Reference Range

AUC*:  80 - 120 mg/hr/L

*Note: AUC is calculated by pharmacy (987-2033) and documented in inpatient progress note.

Critical Values

0 - 1 year:   > 12 mcg/mL

Greater than 1 year:  > 30 mcg/mL