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Test Code LAB2970 Cytokine Panel

Clinical System Name

Cytokine Panel

Description

Quantitative chemiluminescent enzyme immunometric assay for IL6, IL8, IL10 and TNF-alpha. Please contact Chemistry to notify of sample collection when results are needed same day. 

Sample Requirements

Specimen: Whole blood

Container(s):  Lavender/EDTA

Preferred Vol: 2 mL

Minimum Vol:  1 mL

 

Note: Send to Lab within one hour of collection. Heparin is unacceptable.

Processing Instructions

Reject due to:

Spin: Y

Aliquot: Y

Temp: -20 C

Storage location: CRITICAL FROZEN. Separate plasma from cells within 2 hours of collection. Promptly place sample in the CPA freezer, Fz5 rack unless assay will be performed immediately. Call Chemistry (987-2565) when samples are received.

 

Off-site collection:  CRITICAL FROZEN. Separate plasma from cells within 2 hours of collection. Send frozen.

Stability

Specimen Type Temperature Time
Plasma Room temp

1 h
  Refrigerated N
  Frozen

1 y

 

Department

Department:  Chemistry

Phone Number: 206-987-2565

Synonyms

IL 6

IL-6

IL 8

IL-8

IL 10

IL-10

TNF alpha

Cytokines

Availability

STAT Performed TAT
N M-F, dayshift 2 - 5 d

Please contact the Chemistry Department (987-2565) if immediate turn around time is necessary.

Methodology

Method: Quantitative immunoassay using the Siemens Immulite

Analytical Volume: 0.6 mL

Limitations:

Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with in vitro immunoassays. Samples from patients routinely exposed to animals or animal serum products can demonstrate this type of interference potentially causing an anomalous result. These reagents have been formulated to minimize the risk of interference; however, potential interactions between rare sera and test components can occur.

The drug asfotase alfa (STRENSIQ), a recombinant form of alkaline phosphatase, is expected to interere with this assay as this assay utilizes an alkaline phospatase detection system.

Reference Range

 

Analyte Reference Interval
Interleukin 6 0 - 9 pg/mL
Interleukin 8 0 - 116 pg/mL
Interleukin 10 0 - 9 pg/mL
Tumor Necrosis Factor - alpha 0 - 98 pg/mL

 

CPT Codes

83520 x 3

83529

Special Instructions

Please notify the Chemistry  Laboratory (987-2565) of sample collection when same day turn around time is necessary.