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HelpTest Code LAB2979 DNA Antibody (Double-Stranded)
Clinical System Name
Double-stranded DNA (dsDNA) Antibody
Synonyms
Anti DNA Antibodies; Anti double stranded DNA; Anti dsDNA; Anti Native DNA; Anti-DS DNA; DNA Binding; ANTI DNA
Sample Requirements
Specimen: Whole Blood
Container(s): Gold SST, Red, Dark Green/Sodium Heparin, Lavender/EDTA
Preferred Vol: 2.0 mL
Minimum Vol: 1.0 mL
Processing Instructions
Reject due to:
Spin: Y
Aliquot: Y
Temp: 2-8 C
Storage location: CPA Refrigerator, FrigF Rack.
Off-site collection: Avoid hemolysis. Spin blood, refrigerate 1 mL serum/plasma in a polypropylene aliquot tube.
Stability
| Temperature | Time |
|---|---|
| Room temp | |
| Refrigerated | 7 d |
| Frozen | 6 m |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N* | M-F | 1-3 d |
*Available STAT only upon approval of Seattle Children's Hospital Pathologist on call.
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Chemistry
Location: Chem East
Phone Number: (206) 987-2617
CPT Codes
86225
Methodology
Method: Immunoassay on Bioplex 2200
Analytical Volume: 0.4 uL serum
Limitations:
Reference Range
| IU/mL | |
|---|---|
| Negative | < 4 |
| Equivocal | 5-9 |
| Positive | > 10 |
Description
This assay is used in the detection and quantitation of autoantibodies to aid in the diagnosis of autoimmune disease, typically in evaluating patients with signs and symptoms consistent with systemic lupus erythematosis (SLE) and in monitoring patients with documented SLE for flares in disease activity.