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HelpTest Code LAB3031 Factor V Leiden
Clinical System Name
Factor V Leiden Mutation - DNA
Synonyms
Factor 5 Leiden
Factor V Leiden
Factor V PCR
DNA-Factor V
Factor 5 DNA Screen
APC CoFactor-DNA
Description
Sequencing of F5 to analyze genetic causes of factor V Leiden thrombophilia at University of Washington Genetics and Solid Tumors Lab (UW GSTL).
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender/EDTA or Yellow/ACD A or B
Preferred Vol: 3.0 mL (Adult), 2mL (child)
Minimum Vol: 1.0 mL
Specimen: DNA
Container(s): Sterile plastic tube
Preferred Vol: 20-30 ul of 100ng/ul concentration
Note: Only one sample is needed to perform both Factor V Leiden and Prothrombin Variant.
Alternative Specimen (e.g. salvia or buccal): Alternate Specimen Collection Kits for Genetic Testing
Processing Instructions
Reject due to: Dark Green/Sodium Heparin, Lt. Green/Lithium Heparin Microtainer
Spin: N
Aliquot: N
Temp: 2 - 4 C
Storage location: CPA 3 refrigerator, Send Outs rack.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood | Room Temp | 7 d |
| Refrigerated | 7 d | |
| Frozen | Unacceptable | |
| Extracted DNA | Room Temp | 3-4 d |
| Refrigerated | 1 y | |
| Frozen | Indefinitely |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | Weekly | 2 w |
Performing Laboratory
University of Washington Genetics and Solid Tumors Laboratory (UW GSTL)
Department of Laboratory Medicine
UW Genetics Lab
1959 NE Pacific St, NW220
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs/Genetic
Phone Number: (206) 987-2563
Methodology
Next-generation sequencing of exons for F5.
Reference Range
Normal result: no mutations detected.
Clinical Utility
A genetic diagnosis may have implications for treatment, management, recurrence risk, and family member testing.
Send Out Instructions
| Reference Test Name: | Factor V DNA Screen |
| Reference Test Number: | F5DNA |
| Instructions: | Send out Monday through Friday with the UW courier. |
Exclude from UM database