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HelpTest Code LAB3035 Factor 8 Inversion DNA
Additional Codes
F8 Inversion
Clinical System Name
Factor 8 Inversion DNA
Synonyms
Bloodworks Northwest F8 Inversion; DNA Factor VIII Inversion; Intron 1 or 22 Inversion
Sample Requirements
Preferred Specimen: Whole Blood
Container(s): Lavender/EDTA
Preferred Vol: 10.0 mL
Minimum Vol: 3.0 mL
Alternative Specimen: DNA
Container(s): Sterile plastic tube
Preferred Vol: 10 µg of purified DNA at a concentration of at least 50 µg/ml
Minimum Vol: 5 µg of purified DNA at minimum concentration of 50 µg/ml
Alternative Specimen (e.g. salvia or buccal): Not accepted
Note: A Test Requisition must be completed and accompany specimen to lab. Send tubes to lab immediately.
Whole blood shipped at 2-8 C or ambient temperature is acceptable and should arrive in the lab within 48 hours.
Processing Instructions
Reject due to:
Spin: N
Aliquot: N
Temp: RT
Storage location: Give whole blood to Send Outs.
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | Daily | 5-10 d |
Performing Laboratory
Bloodworks Northwest
Hemostasis Reference Laboratory
921 Terry Ave
Seattle WA 98104
Phone: (206) 689-6594
Department
Department: Send Outs/Genetic
Phone: (206) 987-2563
Reference Range
Interpretive report provided.
Methodology
Method: Polymerase Chain Reaction (PCR) Based
Analytical Volume: 5.0 mL Whole Blood
Clinical Utility
In severe hemophilia A, a common recurrent intron 22 gene inversion accounts for ~45% of the cases. A recurrent intron 1 inversion accounts for ~5% of the cases. These two sites are the first to be screened for severe hemophilia A patients.
Send Out Instructions
| Reference Test Name: | DNA Factor VIII Inversion |
| Reference Lab Test Code: | 3250-05 |
| Instructions: | Send sample and requisition to BWNW via Delivery Express courier. Needs to be delivered to BWNW within 48 hours of collection. |