Test Code LAB3050 FLT3-TKD D835/I836 Mutations

Clinical System Name

FLT3-TKD D835/I836 Mutations

Synonyms

FLT3-Tyrosine Kinase Domain Mutations

Description

This assay detects mutations in the FLT3 gene at codons D835/I836 of the tyrosine kinase domain (FLT3 TKD). Evaluation for FLT3 point mutations in the TKD is indicated at diagnosis of acute myeloid leukemia and may provide prognostic information and direct treatment. Positive FLT3 TKD results may be further evaluated by next-generation sequencing to identify the specific mutation upon request. This assay does not detect other point mutations throughout the TKD nor the FLT3 internal tandem duplication (FLT3-ITD).

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA (preferred) or Dk Green/Sodium Heparin

Preferred Vol: 6.0 mL

Minimum Vol: No microtainers,1mL EDTA

Specimen: Bone Marrow

Container(s): Lavender/EDTA (preferred) or Dk Green/Sodium Heparin

Preferred Vol: 1.0 - 2.0 mL

Minimum Vol: No microtainers, 0.5mL EDTA

For more detailed info for bone marrow testing, see separate Bone Marrow Aspirate listing .

Note: Record specimen type and date/time of collection on label. While green top tubes are accepted for testing, there is documentation that heparin can interfere with some PCR assays.

Specimen: Tissue, Fluids or Sorted cells:

Sterile container in RPMI

Formalin-fixed paraffin embedded tissue (FFPE/PET) block

Preferred Vol: 10 unstained

Minimum Vol: 5 curls/Slides: 5 slides

Pathology should be consulted before ordering testing on FFPE specimens

Processing Instructions

Reject due to:

Spin: No

Aliquot: No

Temp: 2 - 4 C

Notes: Affix label to vacutainer. After hours and on weekends, store sample in CPA refrigerated Send Outs rack to be sent next weekday.

Storage location: Store sample in CPA refrigerator Send Outs rack.

Optimal results are achieved when samples are received within 48 hours of collection for bone marrow and peripheral blood.

Stability

Specimen Type	Temperature	Time
	Room temp	24 h
	Refrigerated	72 h
	Frozen	Unacceptable

Availability

STAT	Performed	TAT
N	M, W, and F	2 w

Performing Laboratory

Department:

UW Molecular Hematopathology Laboratory

825 Eastlake Ave E

Seattle, WA, 98195

Phone Number: (206) 288-7060

Department

Department: Send Outs/Genetic

Phone Number: (206) 987-2563

Methodology

Method: PCR amplification of DNA followed by restriction enzyme and capillary electrophoresis for size fractionation

Analytical Volume: 6.0 mL Whole Blood or 1.0 - 2.0 mL Bone Marrow

Limitations: None specified

Special Instructions

UWMC

Requisition

Place test orders in EMR.

For bone marrow, use order "HemOnc Bone Marrow Requisition" or "Hematopoietic Transplant Marrow Requisition", as appropriate.

Send Out Instructions

Reference Test Name:

FLT3-TKD D835/I836 Mutations

Reference Test Number:

FLTTKD

Instructions:

Send Monday through Friday with the UW Courier.