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HelpTest Code LAB3059 Fondaparinux Activity Assay
Additional Codes
Fondaparinux
Clinical System Name
Fondaparinux Activity Assay
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.
If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of a blood transfer device to ensure proper fill.
Specimen should be processed within one hour of collection.
Processing Instructions
Deliver blood to Core Coag bench. Coag Technologist will process.
Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.
Spin: Y
Aliquot: Y
Temp: -70 C
Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volumel. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be trasferred to the Lt Blue/Citrate tube by use of the blood transfer device.
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood | Room Temp |
4 h |
| Plasma | Refrigerated | N |
| Frozen |
1 m |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y, with UW Lab Med Resident (LMR) approval. |
Daily |
1 -3 d |
Note: Samples received before noon run the same day. To get STAT approval, contact the Send Outs department at ext. 7-2563. After hours or on weekends, contact Central Processing at ext. 7-2102.
Performing Laboratory
University of Washington
Department of Laboratory Medicine
Coagulation
1959 NE Pacific St, NW220
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
CPT Codes
85520
Methodology
Method: This method determines a factor Xa inhibitor level by measuring the inhibition of factor Xa cleavage of a chromogenic substrate. Because no exogenous antithrombin is added in this assay, and because antithrombin plays a significant role in factor Xa inhibition with heparin, low molecular weight heparin, or fondaparinux, patients with lower antithrombin levels (<70%) may demonstrate lower anti-Xa activity.
Analytical Volume: 1.0 mL Plasma
Limitations:
Reference Range
| Prophylaxis Following Surgery | |
| For 3 Hours After Dose | 0.39 - 0.50 mcg/mL |
| Trough Level | 0.14 - 0.19 mcg/mL |
| Treatment of DVT | |
| For 3 Hours After Dose | 1.20 - 1.26 mcg/mL |
| Trough Level | 0.46 - 0.62 mcg/mL |
Send Out Instructions
| Reference Test Name: |
Fondaparinux Activity Assay |
| Reference Lab Test Code: | |
| Instructions: |
Send out Monday through Friday with the UW courier. If ordered STAT, send sample on dry ice directly to UW NW220 for testing via Delivery Express. |