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HelpTest Code LAB3059 Fondaparinux Activity Assay
Additional Codes
Fondaparinux
Clinical System Name
Fondaparinux Activity Assay
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Note: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.
Please review the Anticoagulant Job Aid for additional collection instructions for patients with lines.
Processing Instructions: Main Campus
Deliver blood to Core Coag bench. Coag Technologist will process.
Reject due to: Clotted, hemolyzed, insufficient quantity, or improper collection.
Spin: Y
Aliquot: Y
Temp: -70 C
Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.
Specimen should be centrifuged within four hours of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Processing Instructions: Offsite & Regional Clinics
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: -70 C (preferred) or -20 C.
Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.
Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood | Room Temp |
4 h |
| Plasma | Refrigerated | N |
| Frozen |
1 m |
Performing Laboratory
University of Washington
Department of Laboratory Medicine
Coagulation
1959 NE Pacific St, NW220
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
Send Out Instructions
| Reference Test Name: |
Fondaparinux Activity Assay |
| Reference Lab Test Code: | |
| Instructions: |
Send out Monday through Friday with the UW courier. If ordered STAT, send sample on dry ice directly to UW NW220 for testing via Delivery Express. |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y, with UW Lab Med Resident (LMR) approval. |
Daily |
1 -3 d |
Note: Samples received before noon run the same day. To get STAT approval, contact the Send Outs department at ext. 7-2563. After hours or on weekends, contact Central Processing at ext. 7-2102.
Methodology
Method: This method determines a factor Xa inhibitor level by measuring the inhibition of factor Xa cleavage of a chromogenic substrate. Because no exogenous antithrombin is added in this assay, and because antithrombin plays a significant role in factor Xa inhibition with heparin, low molecular weight heparin, or fondaparinux, patients with lower antithrombin levels (<70%) may demonstrate lower anti-Xa activity.
Analytical Volume: 1.0 mL Plasma
Limitations:
Reference Range
| Prophylaxis Following Surgery | |
| For 3 Hours After Dose | 0.39 - 0.50 mcg/mL |
| Trough Level | 0.14 - 0.19 mcg/mL |
| Treatment of DVT | |
| For 3 Hours After Dose | 1.20 - 1.26 mcg/mL |
| Trough Level | 0.46 - 0.62 mcg/mL |
CPT Codes
85520