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HelpTest Code LAB313 D-Dimer
Clinical System Name
D-Dimer
Description
This particular test is used in conjunction with other tests (e.g. Platelet count, Clauss Fibrinogen, Thrombin Time, and to a lesser extent PT and APTT) to identify patients with DIC.
Sample Requirements
Specimen: Whole Blood
Container(s): Lt Blue/Citrate
Required Vol: (1) 1.8 mL or (1) 2.7 mL
Minimum Vol: N
Note: This is enough for a PT, PTT, TT, Fibrinogen, and D-Dimer to be run on the same sample.
Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions.
Processing Instructions: Main Campus
Deliver whole blood to Coag bench. Coag Technologist will process.
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: Core 14 Freezer ( -70 C).
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Do not pool. Assay immediately.
Processing Instructions: Offsite & Regional Clinics
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: -70 C (preferred) or -20 C.
Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.
Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Citrated platelet-poor plasma | Room temp | ≤ 4 h |
| Refrigerated | N | |
| Frozen -20 C or -70 C | ≤ 3 m |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Coagulation
Phone Number: 206 987-2617 (Client Services)
Synonyms
LAB313
D-DIMER
D Dimer
DDimer
FDP
Fibrin Degradation Products
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y |
Daily |
1 h |
Methodology
Method: Latex-binding immunoturbidimetric assay by STA-R MAX
Analytical Volume: 200 mL plasma
Limitations: Method is affected by moderate lipemia, moderate hemolysis, moderate icterus, insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.
Reference Range
≤ 0.5 ug/mL FEU
CPT Codes
85378
Critical Values
Not defined for this test.