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HelpTest Code LAB314 Fibrinogen
Clinical System Name
Fibrinogen
Description
The measurement of fibrinogen is important in the evaluation of 1) a patient suffering from uncontrolled bleeding, 2) one who has received large quantities of transfused blood within a short period of time, 3) one who is in danger of developing DIC as a complication of another disease, or 4) one who has demonstrated an unexplained tendency to bleed. In addition to measuring the quantity of normal fibrinogen, this test (which is kinetic) may be used in conjunction with other methods which yield a total clottable fibrinogen level to establish the presence of abnormal or non-functioning fibrinogen.
Sample Requirements
Specimen: Whole Blood
Container(s): Lt Blue/Citrate
Required Vol: (1) 1.8 mL or (1) 2.7 mL
Minimum Vol: N
Note: This is enough for a PT, PTT, TT, and Fibrinogen to be run on the same sample.
Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions.
Processing Instructions: Main Campus
Deliver whole blood to Coag bench. Coag Technologist will process.
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: Core 14 Freezer ( -70 C).
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Do not pool. Assay immediately.
Processing Instructions: Offsite & Regional Clinics
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: -70 C (preferred) or -20 C.
Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.
Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Citrated platelet-poor plasma | Room temp | ≤4 h |
| Refrigerated | N | |
| Frozen -20 C or -70 C | ≤3 m |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Coagulation
Phone Number: 206 987-2617 (Client Services)
Synonyms
LAB314
FIB
Fib
Fibrinogen
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y |
Daily |
1 h |
Methodology
Method: Electromagnetic mechanical clot detection assay by STA-R MAX
Analytical Volume: 100 uL plasma
Limitations: Method is affected by insufficient quantity (underfill), improper collection (overfill), improper processing, and improper storage. High hematocrit greater than or equal to 56% requires a citrate adjusted tube.
Argatroban levels greater than 3ug/mL may falsely decrease Fibrinogen results more than 18%. Argatroban from 2 to 3ug/mL may falsely decrease Fibrinogen results from 10 to 18%. Argatroban levels less than 2ug/mL do not significantly affect Fibrinogen results.
Reference Range
Full Term Infant - Adult 230 - 450 mg/dL
CPT Codes
85384
Critical Values
< 60 mg/dL.