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HelpTest Code LAB314 Fibrinogen
Clinical System Name
Fibrinogen
Sample Requirements
Specimen: Whole Blood
Container(s): Lt Blue/Citrate
Required Vol: one 1.8 mL tube or one 2.7 mL tube (do NOT over or under fill)
Minimum Vol: N
Note: This is enough for a PT, PTT, TT, and Fibrinogen on the same sample. Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended.
If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be transferred to the Lt Blue/Citrate tube by use of a blood transfer device to ensure proper fill.
Specimen should be centrifuged within 1 hour of collection.
Processing Instructions
Main Campus CPA: Core Technologist will process specimen. Deliver blood directly to Core Coag bench.
Reject due to: Clotted, Insufficient quantity (underfill), or Improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: Core 14 Freezer ( -70 C).
Core Technologist: Specimen should be centrifuged within 1 hour of collection. Spin whole blood, remove plasma. Transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label.
Off-site collection: Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume, note the fill line on the tube. Use of a Vascular Access Device for the collection of coag testing is not recommended. If this method is used it requires a 5 mL waste volume; 3 mL for size 2 French catheter or smaller. Blood must be transferred to the Lt Blue/Citrate tube by use of the blood transfer device.
Specimen should be centrifuged within 1 hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large computer label. Freeze until solid one aliquot of 1.0 mL plasma in plastic tube at -70C (preferred) or -20C.
Non-Children's Hospital Off-site collection: Ship completely frozen on dry ice.
Children's Regional Clinics: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Citrated platelet-poor plasma | Room temp | ≤4 h |
| Refrigerated | N | |
| Frozen -20 C or -70 C | ≤3 m |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Coagulation
Phone Number: 206 987-2617 (Client Services)
Synonyms
LAB314
FIB
Fib
Fibrinogen
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y |
Daily |
1 h |
Methodology
Method: Electromagnetic mechanical clot detection system by STA-R MAX
Analytical Volume: 1 mL plasma
This is enough for a PT, PTT, TT, and Fibrinogen on the same sample.
Limitations: Method is affected by improper collection, processing, and storage.
High hematocrit greater than or equal to 56% requires a citrate adjusted tube.
Argatroban levels greater than 3ug/mL may falsely decrease Fibrinogen results more than 18%. Argatroban from 2 to 3ug/mL may falsely decrease Fibrinogen results from 10 to 18%. Argatroban levels less than 2ug/mL do not significantly affect Fibrinogen results.
Reference Range
Full Term Infant - Adult 230 - 450 mg/dL
Critical Values
Critical Value: Less than 60 mg/dL.
CPT Codes
85384
Description
The measurement of fibrinogen is important in the evaluation of 1) a patient suffering from uncontrolled bleeding, 2) one who has received large quantities of transfused blood within a short period of time, 3) one who is in danger of developing DIC as a complication of another disease, or 4) one who has demonstrated an unexplained tendency to bleed. In addition to measuring the quantity of normal fibrinogen, this test (which is kinetic) may be used in conjunction with other methods which yield a total clottable fibrinogen level to establish the presence of abnormal or non-functioning fibrinogen.