Sign in →

Test Code LAB3140 HCG Preg

Clinical System Name

HCG, Serum Pregnancy Test

Sample Requirements

Specimen: Whole Blood

Container(s):  Gold SST, Red, Gold Microtainer

Preferred Vol:  3.0 mL

Minimum Vol:  0.5 mL


Notes: Method is affected by pregnancy status and certain tumors. Plasma is unacceptable specimen type.

Indeterminate results are reflexed to UW Laboratory STAT for HCG Quant (R/O Preg) testing.

Processing Instructions

Reject due to: Age of specimen, Insufficient Quantity, Wrong specimen type

Spin: Y

Aliquot: Y

Storage location: Core 5 Chemistry Refrigerator.


Off-site collection: Specify: "HCG-R/O Preg." Specimen should be centrifuged as soon as possible after collection. Spin blood and transfer serum to plastic tube and refrigerate or freeze. Serum may only be frozen and thawed ONCE prior to testing.


Specimen Type Temperature Time
Serum RT

≤ 8 h

Serum 2-8 C

≤ 48 h

Serum -20 C or -70 C

≤ 6 m


STAT Performed TAT
Y 24/7 Hospital Lab 1 h
Y 24/7 Reflexed indeterminate results 2 h
Y Reginal Clinic indeterminate results 4 h

Performing Laboratory

Seattle Children's Laboratory    


Department:  Core Chemistry

Phone Number: 206-987-2617 (Client Services)


Method:  Immunochromatographic method for qualitative detection

Analytical Volume:  0.135 mL serum

Reference Range

Pregnancy Confirmed ≥ 10 mIU/mL



The serum hCG test is a rapid immunoassay for the qualitative detection of human chorionic gonadotropin (hCG) in urine or serum as an aid in the early determination of pregnancy. hCG is a glycoprotein hormone secreted by the developing placenta. After fertilization, the concentration of hCG rapidly rises in both the urine and serum of pregnant people, making the detection of hCG in these fluids an excellent marker for confirming pregnancy. The test utilizes monoclonal and polyclonal antibodies to hCG.

CPT Code




HCG Preg