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HelpTest Code LAB3164 Herpes Simplex Western Blot
Additional Codes
HSWB
Description
The detection of HSV1 and HSV2 IgG class antibodies by Western blot in serum or CSF. HSV1 and HSV2 proteins from detergent lysates ("Bernstein's lysate") of HSV infected cells are separated by electrophoresis and transferred to nitrocellulose paper. The strips of paper or "blots" containing separated fixed proteins from either HSV-1 or HSV-2 are incubated with the patient's serum. Antibodies, which bind to the viral proteins, are detected by an enzyme-mediated color change. The pattern of staining on the two blots (HSV-1 and HSV-2) is dictated by the number and identity of the HSV proteins to which the patient's immune system has antibody.
Clinical System Name
Herpes Simplex Western Blot
Synonyms
Herpes 1 + 2 Serology - 1 Serum; Herpes Type-specific Serology; HSV Antibody Status; HSV IgG; HSV Serology - Single Serum; HSV Titers; HSV Type-specific Serology; HSV Western Blot; HSVEIA
Sample Requirements
Specimen Type: Blood
Container Type(s): Gold or Gold Microtainer
Preferred Vol: 2.0 mL
Minimum Vol: 1.0 mL
Processing Instructions
Reject due to:
Spin: Y
Aliquot: Y
Temp: 2 - 8 C
Storage Location: Spin blood, transfer 1.0 mL serum to a plastic aliquot tube affixed with a large Epic label. Place in the CPA refrigerator, Send Outs rack.
Off-site Collection: Spin, aliquot serum, and refrigerate.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Room Temp |
Unacceptable |
| Refrigerated | 7 d | |
| Frozen |
Indefinite |
Availability
| Available STAT? | Performed | TAT |
|---|---|---|
| N | M and Th | 2 - 3 d |
Note: 20% of samples need 5 - 7 days of additional testing to confirm results.
Performing Laboratory
University of Washington
Department of Laboratory Medicine
Virology Lab
1959 NE Pacific St, NW220
Seattle, WA 98195
Phone Number: (206) 520-4600
Department
Department: Send Outs
Phone Number: (206) 987-2563
CPT Codes
86695, 86696
Methodology
Method: Western Blot
Analytical Volume: 0.5 mL Serum
Limitations:
Send Out Instructions
| Reference Lab Test Name: | Herpes Simplex Western Blot |
| Reference Lab Test Number: | |
| Instructions: |
Send out daily with the UW courier. |