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HelpTest Code LAB3174 HLA Antibody Detection
Additional Codes
HLA AB DT
Clinical System Name
HLA Antibody Detection
Synonyms
Panel Reactive Antibodies; PRA; HLA Antibody Screen
Sample Requirements
Specimen: Whole Blood
Container(s): Red
Preferred Vol: 7.0 mL
Minimum Vol: 3.0 mL
Note: NO GEL SEPARATOR TUBES. Do not refrigerate or place on ice. Samples should arrive at the BWNW Immunogenetics/HLA Laboratory within 48 hours of collection. If sample delivery is delayed, call BWNW Immunogenetics/HLA Laboratory to confirm sample acceptability.
Note: 3.0 ml ACD and 3.0 mL Red Top can be shared for HLA Class I,II, Lymphocyte Crossmatch, and HLA Ab Detection when patient is having initial HLA Work-up.
Deliver specimen and BWNW Immunogenetics/HLA Laboratory Requisition to the Lab. Fill out entire form as completely as possible. Information on the label and requisition must match exactly.
Processing Instructions
Reject due to: Gold/SST
Spin: N
Aliquot: N
Temp: RT
Storage Location: Use a BWNW Immunogenetics/HLA Laboratory Requisition. Fill out as completely as possible, making sure the ordering provider is listed. Place a large Epic label in the top right corner and deliver the bagged sample and requisition to the room temperature Send Outs rack.
Note: If order is marked STAT on requisition OR is ordered STAT in CIS, send STAT to BWNW (921 Terry Ave) via Delivery Express.
Off-site collection: Keep whole blood at room temperature and transport to Seattle Childrens.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood in Red Top | Room temp | 10 d |
| Refrigerated | Unacceptable | |
| Frozen | Unacceptable |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M - F | 5 - 7 d |
Performing Laboratory
Bloodworks Northwest
Immunogenetics/HLA Laboratory
921 Terry Ave
Seattle, WA 98104
Phone: (206) 689-6580
Department
Department: Send Outs
Phone: (206) 987-2563
CPT Codes
86828
Methodology
Method: Flow Cytometry using microparticles ("beads") coated with recombinant or soluble HLA antigens
Analytical Volume: See "Specimen Requirements" section.
Limitations:
Reference Range
None specified
Send Out Instructions
| Reference Test Name: |
HLA Antibody Detection |
| Reference Lab Test Code: | 3083-06 |
| Instructions: | Deliver the bagged sample and requisition to the room temperature Send Outs rack. |