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HelpTest Code LAB3178 HLA Specific Antibody Monitoring
Additional Codes
HLA Ab Mon
Clinical System Name
HLA Specific Antibody Monitoring
Synonyms
Donor Specific Antibody; DSA
Sample Requirements
Specimen: Whole Blood
Container(s): Red (Clotted)
Preferred Vol: 5.0 - 7.0 mL
Minimum Vol: 3.0 mL
Note: NO SERUM SEPARATOR TUBES. A BWNW Immunogenetics/HLA Laboratory Requisition must be complete and accompany specimen to lab. Do not use serum separator tubes. Do not refrigerate or place on ice. Samples must arrive at BWNW Immunogenetics/HLA Lab within 10 days from the date of collection. If sample delivery is delayed, contact BWNW Immunogenetics/HLA Lab to confirm specimen acceptability.
Processing Instructions
Reject due to: Gold/SST, Serum separator gel tube
Spin: N
Aliquot: N
Temp: RT
Storage Location: Use a BWNW Immunogenetics/HLA Laboratory Requisition. Fill out as completely as possible, making sure the ordering provider is listed. Remove "Originator/Hospital" copy; place a large Cerner label in the top right corner. Deliver the bagged sample and requisition to the room temperature Send Outs rack.
Note: If order is marked STAT on requisition OR is ordered STAT in CIS; send STAT to BWNW (921 Terry Ave) via Delivery Express.
Off-site collection: Keep whole blood at room temperature and transport to Seattle Children's.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood | Room temp | 15 d |
| Refrigerated | Unacceptable | |
| Frozen | Unacceptable |
Availability
| STAT | Performed | TAT |
|---|---|---|
| M - F | 2 - 4 d (Routine) | |
| Y | Daily | 5 - 10 h (STAT) |
For specimens to be processed immediately at BWNW Immunogenetics Lab, SCH provider must call Bloodworks Northwest Immunogenetics Lab for approval, otherwise STAT specimen will be performed on next run.
Performing Laboratory
Bloodworks Northwest
Immunogenetics HLA Laboratory
921 Terry Ave
Seattle, WA 98104-1256
Phone: (206) 689-6580
Department
Department:
Bloodworks Northwest
Immunogenetics HLA Laboratory
921 Terry Ave
Seattle, WA 98104-1256
Phone number:
(206) 689-6580
CPT Codes
86834, 86835
Methodology
Method: Flow Cytometry using microparticles ("beads") coated with recombinant or soluble HLA antigens
Analytical Volume: See "Specimen Requirements" section.
Limitations:
Reference Range
None specified
Send Out Instructions
| Reference Test Name: | HLA Antibody Specificity-Monitoring (DSA Donor-Specific Antibody) |
| Reference Lab Test Code: | 3083-17 (Routine) or 3083-23 (Protocol) |
| Instructions: |
Deliver the bagged sample and requisition to the room temperature Send Outs rack. If order is marked STAT on requisition OR is ordered STAT in CIS; send "RUSH" to BWNW (921 Terry Ave) via Delivery Express. |
Special Instructions
If results are required within 12 hours, the Monitoring HLA Specific Antibody – STAT transaction code (3083-18) applies instead.