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Test Code LAB3179 HLA Typing FHCC

Important Note

This test requires Red AND Dark Green (NaHep). Call HLA Lab to schedule and get draw volumes at (206) 606-7700. A completed Fred Hutch Requisition for HLA Testing is required (completed by the care team). Please complete the form and send it with sample(s) to SCH Main Lab.

On Fridays, specimen MUST arrive in lab by 1200.

Clinical System Name

HLA Typing FHCC

Sample Requirements

Specimen: Whole Blood

Container(s): Red AND Dark Green/Sodium Heparin

Preferred Vol: See note

Minimum Vol: Sample requirements may vary. Call the FHCC HLA Lab at (206) 606-7700 for information when scheduling test.

 

Note: Prior to collection, the disease-based team should e-mail mail BMTIntake@seattlechildrens.org with patient identifiers for patient requiring HLA testing. Once received, BMT Intake team will perform patient screening, place external referral orders, and communicate with Fred Hutchinson Cancer Center (FHCC) teams according to Job Aid: RNC Flow for HLA Only Orders at FHCC, 14216. FHCC is then able to place internal orders to the FHCC Clinical Immunogenetics Laboratory (CIL) to process the typing.


For collection, the collecting RN should call FHCC CIL at (206) 606-7700 if the required lab volumes are not listed on the paper lab requisition. Green top tubes with powdered Na Heparin are okay to use. A Fred Hutch Requisition for HLA Testing should be completed and sent to Seattle Children’s Main Lab with sample(s). 


Upon approval from FHCC, buccal swabs can be obtained from the SCH Main Lab. Please call the Central Processing Area (CPA) at ext. 7-2727 to acquire FHCC buccal swabs.

Processing Instructions

Reject due to: Gold SST

Spin: N

Aliquot: N

Temp: RT

Storage location: Verify that the sample is labeled with the first and last name of the person from whom it was obtained and the date of collection. Give blood and FHCC Requisition For HLA Testing form to Send Outs.

 

Off-site Collection: Verify that the requisition is complete. Only fill out the phlebotomist verification section. If the requisition is not complete, page the ordering provider. Call the HLA Lab for specimen requirements and Send Outs with other questions.

Stability

Specimen Type Temperature Time
Whole Blood Room temp

3 d

  Refrigerated N
  Frozen

N

 

Performing Laboratory

Fred Hutch Cancer Center

Clinical Immunogenetics Laboratory

188 E. Blaine St. Suite 250

Specimens: Room 2120

Seattle, WA 98102

 

Phone Number: (206) 606-7700

Department

Department: Send Outs

Phone Number: (206) 987-2563

Synonyms

HLA Typing FHCC; Tissue Typing

Availability

STAT Performed TAT
N Cannot be drawn on Saturdays, or on Fridays after 1200 30 d

 

Methodology

Method: None Specified

Analytical Volume: Call FHCC HLA Lab for minimum volumes at (206) 606-7700.

Limitations:

Send Out Instructions

Reference Test Name: N/A
Reference Test Number:

N/A

Instructions:

Verify specimen labeling. Check Fred Hutch Requisition for HLA Testing forms for completeness; correct if necessary. Scan the original to Send Outs and send the original form with the blood/swabs to the FHCC CIL/ HLA Lab.