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Test Code LAB318 Platelet Function Screen

Important Note

Hour of availability are M - F (05:00 - 21:00) at Main Campus ONLY.  No holidays or weekends. Requests outside hours of availability must be directed to the Lab Leader on Call before the sample is collected.

Night shift personnel are not trained to perform this test.

Performed at Seattle Children's Main Campus only. Do NOT collect at Seattle Children's Regional Clinic locations. 

 

Do NOT use tube system to transport samples. Samples must be hand carried to the laboratory. Do not agitate the sample. Testing must be performed within 4 hours of collection. Sample cannot be used for any other testing.

Clinical System Name

Platelet Function Screen

Synonyms

LAB318

PLT FUNC S

PFS
PLT FUNC S
PLT Function Screen

Sample Requirements

Specimen: Whole blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (4) 2.7 mL tubes - please read instructions in note before drawing.

Minimum Required Vol:  (3) 2.7 mL tubes - please read instructions in note before drawing.

 

Note: Do NOT use tube system to transport samples. Samples must be hand carried to the laboratory. Do not agitate the sample. Testing must be performed within 4 hours of collection. Sample cannot be used for any other testing.

 

Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use 23 gauge or larger needle. Draw blood directly into vacutainer tubes, no syringe draws. Invert sample 4-5 times only.

Use of a Vascular Access Device for the collection of coag testing is not recommended.

If this method is used it requires a 5 cc clearing volume; 3 cc for size 2 French catheter or smaller. Blood must be transferred to the Lt. Blue/Citrate tube by use of a blood transfer device to ensure proper fill. 

Processing Instructions

Reject due to:

  • Hemolyzed, clotted, or inadequately filled samples are rejected.
  • Specimen tubes sent to the lab via the pneumatic tube transport system.
  • Specimen tubes that have been refrigerated, centrifuged, or greater than 4 hours old.

 

Spin: N

Aliquot: N

Storage location: Core 5 Fridge

 

Note: Deliver blood to Core Coag bench (next to TEG rack). Coag Technologist will process. 

Do not centrifuge specimen. Test should be set up within 4 hours of collection.

 

Off-site collection: Cannot be collected outside Seattle Children's Main Campus. Do NOT collect at Seattle Children's Regional Clinic locations. Contact Laboratory for additional information.

Stability

Specimen Type Temperature Time

Whole blood

Lt Blue/Citrate

 Room temp ≤4 h
  Refrigerated N
  Frozen N

 

Availability

STAT Performed TAT
N M-F  (07:00 - 19:00)  No holidays or Weekends 1 d

 

 Requests outside these hours must be directed to the Lab Leader on Call before the sample is collected.

Performing Laboratory

Seattle Children's Laboratory    

 

Department

Department:  Coagulation

Phone Number: 206 987-2617 (Client Services)

 

 

CPT Codes

85576

Methodology

Method:  Test cartridge system by Dade® PFA-100 Instrument.

 

Analytical Volume: 1.8 mL Whole blood

 

Limitations: Method is affected by improper handling. Do not use tube system to transport samples. Do not agitate samples. Testing must be performed within 4 hours.

 

Reference Range

  Seconds

Collagen/ADP Occlusion Time

 ≤ 114

Collagen/EPI Occlusion Time 　

 ≤ 180

 

Description

The PFA-100 can be used to detect platelet dysfunction. Platelet dysfunction may be acquired, inherited, or the result of platelet inhibiting agents. The most common causes of platelet dysfunction are related to uremia, von Willebrand disease, and exposure to medications such as acetylsalicylic acid (aspirin) and clopidogrel (plavix).

Critical Values

Not defined for this test.