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HelpTest Code LAB3220 Inhibin B, Serum
Reporting Name
Inhibin B, SSCH Clinical System Name: Inhibin B Level
Useful For
Aiding in the diagnosis of granulosa cell tumors and mucinous epithelial ovarian tumors
Monitoring of patients with granulosa cell tumors and epithelial mucinous-type tumors of the ovary known to overexpress inhibin B
As an adjunct to follicle-stimulating hormone testing during infertility evaluation
Ordering Guidance
For the initial evaluation of patients suspected of having a granulosa cell tumor of the ovary, order INHAB / Inhibin A and B, Tumor Marker, Serum. If the results of the profile show that either inhibin A or B are elevated, consider monitoring the patient with the individual tests, INHA / Inhibin A, Tumor Marker, Serum or INHB / Inhibin B, Serum.
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Collect 2.0 mL (Min. 1.2 mL) whole blood in a Red Top-Plain.
Specimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 90 days | ||
| Ambient | 7 days |
Day(s) Performed
Monday, Wednesday, Friday
TAT: 3 - 8 days
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Method Description
The ultra-sensitive inhibin B enzyme-linked immunosorbent assay is a quantitative three-step sandwich type immunoassay. Sample is incubated in wells that have been coated with inhibin B antibody. After incubation and washing, the wells are incubated with biotinylated inhibin B antibody. After a second incubation and washing step, the wells are incubated with streptavidin horseradish peroxidase conjugate. After the third incubation and washing step, the wells are incubated with substrate solution. After incubation, an acidic stopping solution is added. Antibody-analyte complex is detected by dual wavelength absorbance measurement at 450nm as the primary test filter and 620 nm as the reference filter. The absorbance measured is directly proportional to the concentration of inhibin B in the samples and calibrators.(Unpublished Mayo method)
Reference Values
Males
<15 days: 68-373 pg/mL
15-180 days: 42-516 pg/mL
6 months-7 years: 24-300 pg/mL
8-30 years: 47-383 pg/mL
31-72 years: <358 pg/mL
>72 years: Not established
Females
≤12 years: <183 pg/mL
13-41 years Regular Cycle (Follicular Phase): <224 pg/mL
42-51 years Regular Cycle (Follicular Phase): <108 pg/mL
13-51 years Regular Cycle (Luteal Phase): <80 pg/mL
>51 years (Postmenopausal): <12 pg/mL
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
83520
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| INHB | Inhibin B, S | 56940-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 88722 | Inhibin B, S | 56940-0 |
Report Available
2 to 4 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Forms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.