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Test Code LAB3231 Intraoperative Parathyroid Hormone (PTH)

Important Note

Call Chemistry at x7-2565 when sample is received in lab and hand to Special Chemistry for expedited processing.

Microtainers used for capillary collections are unacceptable. 

 

Clinical System Name

Intraoperative PTH, Intact

Synonyms

PTH Intraop

ioPTH

 

Sample Requirements

Specimen: Whole blood

Container(s): Collect INTRAOPERATIVE PTH specimens in a DARK GREEN/SODIUM HEPARIN TUBE for expedited processing.  Also acceptable Gold SST, Red, Lt. Green/Lithium Heparin Microtainer, Lavender/EDTA

Preferred Vol: 2.0 mL

Minimum Vol: 1.0 mL

Note:  Microtainers used for capillary collections are unacceptable. If a series of PTH are being drawn, use the same tube type for all collections. 

 

Processing Instructions

Reject due to: capillary collections

Spin: Y

Aliquot: Y

Temp: - 20 C

Storage location: Spin blood within one hour of collection; transfer a minimum of 0.5 mL serum/plasma to aliquot container.

Call Chemistry Lab at x72565 when sample is received.

 

Off-site collection: N/A

Stability

Specimen Type Temperature Time
serum/plasma Room temp 2 h
  Refrigerated 2 d
  Frozen  6 mo

 

Availability

STAT Performed TAT
Y M - F same day

Order comment printed on surgery requisition:  Call Chemistry Lab at x72565 when sample is collected. Send with OR runner to Forest B Level 2 Lab.

Performing Laboratory

Seattle Children's Laboratory    

 

Department

Department:  Chemistry

Location: Chem West

Phone Number: (206) 987-2617

 

 

 

CPT Codes

83970

Methodology

Method:  "intact PTH" chemiluminescent microparticle immunoassay by Alinity i

Analytical Volume: 0.3 mL serum/plasma

Limitations: 

PTH exists as a single chain 84 amino acid polypeptide, numbered from the amino terminal. After secretion, PTH undergoes extensive fragmentation. Only the fragments that carry the amino terminus are biologically active. The half life of active PTH is approximately 5 minutes. Inactive PTH (large C-terminal; PTH 7-84) has a longer half-life; approximately 10 minutes, and smaller C-terminal fragments have even longer half lives. This test and other immunoassays detect the intact PTH molecule (PTH 1-84), along with the large PTH fragment (PTH 7-84) that builds up in renal failure. Smaller PTH  fragments and PTHrP do not cross react.

Reference Range

PTH transitioned platforms from Ortho Vitros 3600 to Abbott Alinity i on August 8, 2022.

There is a 30% positive bias with the new method.

9 - 88 pg/mL