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Test Code LAB3231 Intraoperative Parathyroid Hormone (PTH)

Important Note

Call Core Chemistry at x7-2561 when sample is collected. Once received in lab hand to Core Chemistry for expedited processing.

Microtainers used for capillary collections are unacceptable. 

 

Clinical System Name

Intraoperative PTH, Intact

Sample Requirements

Specimen: Whole blood

Container(s): Collect INTRAOPERATIVE PTH specimens in a Lt. Green/Lithium Heparin tube for expedited processing.  Also acceptable Gold SST, Red, Dark Green/Sodium Heparin tube 

Preferred Vol: 2.0 mL

Minimum Vol: 1.0 mL

Note:  Microtainers used for capillary collections are unacceptable. If a series of PTH are being drawn, use the same tube type for all collections. 

 

Processing Instructions

Reject due to: capillary collections

Spin: Y

Aliquot: Y

Temp: 2-8 C

Storage location: Core 5 

Deliver immediately to Core Chemistry when sample is received.

 

Off-site collection: N/A

Stability

Specimen Type Temperature Time
serum/plasma Room temp 2 h
  Refrigerated 2 d
  Frozen  6 mo

 

Performing Laboratory

Seattle Children's Laboratory    

 

Department

Department: Core Chemistry

Phone Number: (206) 987-2617 (Client Services)

 

 

 

Synonyms

PTH Intraop

ioPTH

 

Availability

STAT Performed TAT
Y 24/7 1 h

Call Core Chemistry Lab at x72561 when sample is collected. Send with OR runner to Forest B Level 2 Lab.

 

Methodology

Method:  "intact PTH" chemiluminescent microparticle immunoassay by Alinity i

Analytical Volume: 0.3 mL serum/plasma

Limitations: 

PTH exists as a single chain 84 amino acid polypeptide, numbered from the amino terminal. After secretion, PTH undergoes extensive fragmentation. Only the fragments that carry the amino terminus are biologically active. The half life of active PTH is approximately 5 minutes. Inactive PTH (large C-terminal; PTH 7-84) has a longer half-life; approximately 10 minutes, and smaller C-terminal fragments have even longer half lives. This test and other immunoassays detect the intact PTH molecule (PTH 1-84), along with the large PTH fragment (PTH 7-84) that builds up in renal failure. Smaller PTH  fragments and PTHrP do not cross react.

Reference Range

9 - 88 pg/mL

CPT Codes

83970