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Test Code LAB3241 JC Virus PCR Quantitation

Additional Codes


Clinical System Name

JC Virus PCR Quantitation


JC Virus; PML Virus; Progressive Multifocal Leukoencephalopathy Virus

Sample Requirements

Specimen: Whole Blood or CSF

Container(s): Lavender/EDTA, Red, or Gold SST OR CSF Tube

Preferred Vol: 3.0 mL Whole Blood or 1.0 mL CSF

Minimum Vol: 1.0 mL Whole Blood or 0.5 mL CSF


Note: This test can share a tube with other Viral PCR testing, but a separate tube needs to be collected if Hematology (CBC, Platelet, ESR, etc) testing is ordered.

Processing Instructions

Reject due to: Plasma collected in Dark Green/Sodium Heparin or Lt. Green/Lithium Heparin.

Spin: Y

Aliquot: Y

Temp: -20 C

Storage Location: CPA -20 freezer, UW rack.


Plasma and CSF:

  • Monday – Friday: Place sample in the CPA -20 freezer, UW rack.
  • Saturday - Sunday & Holidays: Close batch in MayoACCESS and courier to UW Virology.

Off-site Collection:

  • Whole Blood: Centrifuge and transfer 1.5 mL plasma (Min. 0.5 mL) to a plastic aliquot tube affixed with a large Epic label.  Freeze at -20 C.
  • CSF: Freeze within TWO hours of collection. If CSF has cellular material, centrifuge and aliquot clear CSF. Freeze at -20 C.


Specimen Type Temperature Time
Plasma or CSF Room Temp

2 h

  Refrigerated 48 h




STAT Performed TAT
N T 1 - 2 d


Performing Laboratory

University of Washington

Department of Laboratory Medicine

Virology, Eastlake (EVIR)

1959 NE Pacific St, NW220
Seattle, WA 98195


Phone Number: (206) 520-4600


Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes



Method: Real-time Polymerase Chain Reaction (PCR)

Analytical Volume: 0.5 mL Plasma or 0.5 mL CSF


Reference Range

See report

Send Out Instructions

Reference Test Name: JC Virus by PCR Quantitative
Reference Test Code:



Send out daily with the UW courier