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Test Code LAB3268 Luteinizing Hormone (LH), Pediatrics, Serum

Important Note

This test is only performed on patients ≤17 years old at Mayo Medical Laboratories. Please order Luteinizing Hormone (LAB87) for patients ≥ 18 years old.

Additional Codes

LH US

Useful For

Diagnosis of precocious puberty and delayed puberty in children

Method Name

Chemiluminescent Immunoassay (CIA)

Reporting Name

LH, Pediatrics, S
Seattle Children's Hospital Note:

SCH Clinical System Name: Luteinizing Hormone Ultra Sensitive

Specimen Type

Serum


Ordering Guidance


This test is only available for pediatric patients. If testing request is for a patient 18 years of age or older, order LH / Luteinizing Hormone (LH), Serum.



Specimen Required


Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.25 mL

Collection Instructions:

1. Red-top tubes should be centrifuged, and the serum transferred to a plastic vial within 2 hours of collection.

2. Serum gel tubes should be centrifuged within 2 hours of collection.


Seattle Children's Hospital Note:

Collect 1.0 mL whole blood (Min. 0.5 mL) in Red Top-Plain, Gold, or Gold Microtainer.

Specimen Minimum Volume

0.13 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  90 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

Females

<1 year: <0.02-18.3 IU/L

1-8 years: <0.02-0.3 IU/L

9-10 years: <0.02-4.8 IU/L

11-13 years: <0.02-11.7 IU/L

14-17 years: <0.02-16.7 IU/L

 

Tanner Stages*

Stage I (1-8 years): <0.02-0.3 IU/L

Stage II: <0.02-4.1 IU/L

Stage IIl: 0.6-7.2 IU/L

Stage IV-V: 0.9-13.3 IU/L

*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (± 2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African-American girls. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.

 

Males

<1 year: <0.02-5.0 IU/L

1-8 years: <0.02-0.5 IU/L

9-10 years: <0.02-3.6 IU/L

11-13 years: 0.1-5.7 IU/L

14-17 years: 0.8-8.7 IU/L

 

Tanner Stages*

Stage I (1-8 years): <0.02-0.5 IU/L

Stage II: 0.03-3.7 IU/L

Stage IIl: 0.09-4.2 IU/L

Stage IV-V: 1.3-9.8 IU/L

*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (± 2) years. For boys there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.

Method Description

The AnshLite LH CLIA is a quantitative three-step sandwich type immunoassay. In the first step calibrators, controls, and unknown samples are added to luteinizing hormone (LH) antibody-coated microtiter wells and incubated. After washing, the wells are incubated with biotinylated LH antibody solution. After the second incubation and washing, the wells are incubated with streptavidin horseradish peroxidase conjugate solution. Finally, the antibody-antigen and conjugate complex bound to the well is detected by addition of a luminogenic substrate (AnshLite chemiluminescence substrate solution). The relative light output units are directly proportional to the concentration of LH in the samples.(Unpublished Mayo method)

Day(s) Performed

Monday, Wednesday, Friday

Report Available

2 to 6 days
Seattle Children's Hospital Note:

Estimated TAT: 4 - 8 d

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83002

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LHPED LH, Pediatrics, S 83103-2

 

Result ID Test Result Name Result LOINC Value
62999 LH, Pediatrics, S 83103-2
Seattle Children's Hospital Additional Information:

Aliases: Third Generation LH assay; Ultrasensitive LH assay; Lutropin