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HelpTest Code LAB3269 Lyme Disease Serology, Serum
Additional Codes
LYME
Reporting Name
Lyme Disease Serology, SSCH Clinical System Name: Lyme Disease Ab - Reflex to WB IgG/IgM
Useful For
Diagnosing Lyme disease
This test should not be used as a screening procedure for the general population.
This test should not be used for treatment monitoring.
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Information: Centrifuge and aliquot serum into plastic vial.
Collect 1.0 mL whole blood in a Red or Gold top.
Specimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 10 days |
| Frozen | 30 days |
Day(s) Performed
Monday through Friday
TAT: 3 - 6 days
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Method Description
The first-tier Lyme disease screening enzyme-linked immunosorbent assay (ELISA) is the Zeus ELISA Borrelia VlsE1/pepC10 IgG/IgM test system. The Zeus ELISA Borrelia VlsE1/pepC10 IgG/IgM test system is designed to detect IgG- and IgM-class antibodies (not differentiated by the assay in the final result) in human sera to VlsE1 and pepC10 antigens. Diluted test sera are incubated in antigen coated microwells. Any antigen-specific antibody in the sample will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components. Peroxidase conjugated goat antihuman IgG and IgM are added to the wells and the plate incubated. The conjugate will react with IgG and IgM antibodies immobilized on the plate. The wells are washed to remove unreacted conjugate. The microwells containing immobilized peroxidase conjugate are incubated with peroxidase substrate solution. Hydrolysis of the substrate by peroxidase produces a color change. After a period of time the reaction is stopped and the color intensity of the solution is measured photometrically.(Package insert: Borrelia VlsE1/pepC10 IgG/IgM Test System. Zeus Scientific, Inc; Rev. date 05/2021)
Reference Values
Negative
Reference values apply to all ages.
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86618
86617 x 2-Lyme disease confirmation (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LYME | Lyme Disease Serology, S | 20449-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| LYME | Lyme Disease Serology, S | 20449-5 |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| LYWB | Lyme Disease Ab, Immunoblot, S | Yes | No |
Testing Algorithm
If Lyme disease serology is positive or equivocal, then Lyme disease antibody confirmation (by Western blot) will be performed at an additional charge.
Report Available
1 to 4 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat inactivated | Reject |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)
Aliases: Borrelia burgdorferi; B. burgdorferi; Lymes; Lyme C6 Peptide Assay; Tick-borne; Tick; VlsE1/pepC10 Assay