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HelpTest Code LAB3271 Leukocyte Adhesion Defect Panel
Clinical System Name
Leukocyte Adhesion Defect Panel
Description
The leukocyte adhesion panel is used to evaluate for the presence or absence of key molecules on white blood cells that are necessary for their migration out of peripheral blood circulation and into the tissues.
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender Top Tube; Dark Green NaHep also acceptable from referring labs if CBC & differential results submitted with it.
Preferred Vol: 2.0-3.0 mL
Minimum Vol: 2.0 mL; no microtainers
| Collected in-house at Seattle Children's | Collected off-site (including SCH Bellevue, SCH North and SCH South) | |
|---|---|---|
| M-Th | collect any time | collect any time |
| Friday | collect any time | ok if received at SCH main lab same day, if not do not collect |
| Saturday | ok if collected before 12 noon | do not collect |
| day before a holiday | ok if collected before 12 noon | must be received at SCH main lab before 12 noon |
| Sunday | ok if collected after 12 noon (to be tested next morning) |
ok if collected after 12 noon (to be tested next morning) |
| Sunday before a holiday Monday | do not collect | do not collect |
| Holiday | ok if collected after 12 noon (to be tested next morning) | ok if collected after 12 noon (to be tested next morning) |
Children's Outpatient Blood Draw areas and Community Services - order "Lymphocyte Subset" and select Paper Requisition under order comments (specific tests will be ordered in Cell Markers Lab) and submit copy of requisition with specimen.
Processing Instructions
Reject due to: clotted specimen, capillary collection, refrigerated
Spin: N
Aliquot: N
Temp: RT - DO NOT REFRIGERATE
Note: Request Core Lab perform a CBC with differential and ensure a copy of the results is sent with the specimen to Cell Markers. Shared specimens with CBC/Diff orders do not need to repeat testing, but a copy of the results should be printed to accompany the specimen to the Cell Markers Lab. Lavender top Community Service specimens with external CBC Diff results should still be processed by Core for an in-house CBC Diff for comparison if within stability
Storage location: Days: Transport specimen, copy of community service requisition (if applicable), and labels to the Cell Markers Lab (station #181). Eves/Nights: Store specimen, copy of community service requisition (as applicable), and labels in the Cell Markers RT box in CPA.
Off-site collection: Keep whole blood at room temperature. Referring labs may send CBC w/differential results from the same collection along with the sample. Transport specimens to Seattle Children's Hospital address on requisition: Laboratory FB.2.441, 4800 Sand Point Way NE, Seattle, WA 98105.
Stability
| Temperature | Time |
|---|---|
| Room temp |
Lavender Top Tube - 30 hours Dark Green NaHep - 48 hours |
| Refrigerated | N |
| Frozen | N |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Cell Markers
Phone Number: 206-987-2560
Synonyms
Leukocyte Adhesion Defect Full Panel
Lymphocyte Subset
Lymphocyte Subset Analysis
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M-F, Sa (0730-1200) | 2-3 d |
Note: limited specimen stability - see Sample Requirements
Methodology
Method: Flow Cytometry
Analytical Volume: Dependent on patient's white count and testing requested. If <1 ml is collected please ask to recollect in order to obtain minimum volume of 1 ml. If unable to recollect and sample volume is <0.350 ml (350 ul) please cancel and do not send to Cell Markers for testing. If unable to recollect and sample is between 0.350 ml and 1 ml (350 ul-1 ml) please send to Cell Markers, but let patient and/or patient family know that there is a strong possibility that we will not be able to perform the testing due to low volume. We will do our best and understand that the volume needed is dependent on the white count and the specific antibodies that are ordered. (The higher the white count the less volume needed. The more antibodies ordered the more volume needed.)
Reference Range
Reference values accompany patient report.
CPT Codes
| Leukocyte Adhesion Defect Full Panel | Panel Information | CPT CODE(S) |
|---|---|---|
| CM AB IF CD18, CD11 A, B, & C | LAD - CD11a, CD11b, CD11c, CD18 | 86356 |