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HelpTest Code LAB3317 Mitogen Stimulation Study
Clinical System Name
Mitogen Stimulation Study
Synonyms
Anti CD3 Stimulant
Lymphocyte Function Analysis
Mitogen Stimulation Study
PHA (Phytohemaglutinin) Stimulant
T cell proliferation to mitogens
Lymphocyte Mitogen Stimulation
MSS
Sample Requirements
Specimen: Whole Blood
Container(s): Dark Green/Sodium Heparin (no serum separator)
Preferred Vol: 10 mL
Minimum Vol: 10 mL (if unable to collect 10 mL, please contact lab at 206-987-2560)
Note:
Specimens stable for only 24 hours at room temperature.
| Collected in-house at Seattle Children's | Collected off-site (including SCH Bellevue, SCH North and SCH South) | |
|---|---|---|
| M-Th | collect any time | Collect after noon, unless sample reaches Cell Markers lab before noon for same day test set-up. |
| Friday | must be received before 12 noon Friday | must be received at SCH main lab before 12 noon Friday |
| Saturday | do not collect | do not collect |
| day before a holiday | ok if collected before 12 noon | must be received at SCH main lab before 12 noon day before holiday |
| Sunday | ok if collected after 12 noon (to be tested next morning) |
ok if collected after 12 noon (to be tested next morning) |
| Sunday before a holiday Monday | do not collect | do not collect |
| Holiday | ok if collected after 12 noon (to be tested next morning, if Friday Holiday DO NOT COLLECT) | ok if collected after 12 noon (to be tested next morning, if Friday Holiday DO NOT COLLECT) |
Call the Cell Markers Lab (206) 987-2560 with any questions regarding specimen collection or handling. If unable to collect minimum volume, please collect as close to 10 mL as possible (10 mL each for Mitogen and Antigen) and notify Cell Markers at 206-987-2560 (leave voicemail after hours).
Draw an additional 10 mL NaHep if an antigen stimulation studies (candida, tetanus) are also ordered.
Processing Instructions
Reject due to: n/a - send to lab
Spin: N
Aliquot: N
Temp: RT
Storage location: Days: Transport specimen, copy of community services requisition (if applicable), and labels to the Cell Markers lab (station #181). Notify Cell Markers at x72560 right away if specimen is near 24 hour specimen stability limit - billing/ordering problems should not delay delivery to lab. Eves/Nights: Store specimen, copy of community services requisition (if applicable), and labels in the Cell Markers RT box in CPA.
Off-site collection: Dark Blue Na Heparin okay. A normal control should accompany the specimen. It is critical samples be kept at room temperature; use extra packing to maintain temperature. If specimen will not arrive at Children's before noon for same day testing, blood should be drawn as late in the day as possible, maintained at room temperature, and sent overnight to allow test set-up the following morning. Testing must be set up within 24 hours of collection. Transport all tubes at RT to Seattle Children's Hospital address on requisition: Laboratory FB.2.441, 4800 Sand Point Way NE, Seattle, WA 98105.
Stability
| Temperature | Time |
|---|---|
| Room temp | 24 hours |
| Refrigerated | N |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N |
Monday - Thurs; Friday must be in lab before noon |
7 - 10 days |
Limited specimen stability - see sample requirements
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Cell Markers
Phone Number: 206-987-2560
CPT Codes
| CPT CODE | |
|---|---|
| Set up and Initial Mitogen or Antigen Stimulant |
86353 |
| Each additional Mitogen or Antigen Stimulant | 86353 |
Methodology
Method: Lymphocyte proliferation with 3H thymidine incorporation
Analytical Volume: dependent on patient lymphocyte count
Limitations: None specified
Reference Range
Reference values accompany patient report.
Description
The Mitogen Stimulation Study is used in the evaluation of immunodeficiency to determine the functional capabilities of peripheral blood mononuclear cells to respond to non-specific stimuli (PHA and/or anti-CD3).