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Test Code LAB3345 Neuroblastoma Loss of Heterozygosity

Important Note

Pre-treatment tumor samples are strongly preferred.

Required Requisition

If submitting a tumor sample collected during or after treatment (e.g. post-chemotherapy), please indicate post-treatment status of the sample on the requisition form.

Clinical System Name

Neuroblastoma Loss of Heterozygosity

Synonyms

Neuroblastoma LOH

Sample Requirements

Specimen: Tumor

Container(s): Sterile Screw-Capped Container

Preferred Vol: 0.05 g of snap‐frozen tumor tissue is preferred.

Alternative: OCT‐embedded or paraffin embedded tumor tissue (tissue scrolls or paraffin block) also accepted.

Note: At least 40% tumor must be present in the submitted sample (based on internal pathology review). Snap-frozen tissue is preferred.

 

Nationwide Children's Test Information

Processing Instructions

Reject due to: Delayed or improper handling; Insufficient tumor content in tumor sample; Inadequate tissue; Tissue degradation; Wrong type of specimen

Spin: N

Aliquot: N

Temp: -70 C for Tumor Biopsy

Storage Location: Deliver tumor biopsy to the CPA -70 freezer, Send Outs rack.

 

Off-site collection:

Stability

Specimen Type Temperature Time
Paraffin Block Room Temp 6 m
Tissue Scrolls (FFPE) Room Temp 6 m
Tumor Room Temp Unacceptable
  Refrigerated Unacceptable
  Frozen 24 h

 

Availability

STAT Performed TAT
N Monday to Friday 3 w

 

Performing Laboratory

Nationwide Children's Hospital

Nationwide Children's Laboratory Services
700 Children’s Drive, Room C1955
Columbus, OH 43205

 

Phone Number:

Laboratory Client Services
 (614) 722-5477

Department

Department: Send Outs/Genetic

Phone Number: (206) 987-2563

Methodology

This test evaluates for the following clinically significant aberrations within the cancer genome for Neuroblastoma:

  • Copy number loss of 1p, 3p, 4p and 11q
  • Copy number gain of 1q, 2p and 17q
  • Loss of Heterozygosity involving 1p, 3p, 4p and 11q
  • Copy number gain of whole chromosomes will also be detected with the assay

Information regarding other chromosome regions will not be reported, as their clinical significance in neuroblastoma is currently unknown.

Reference Range

Interpretive report is provided

Special Instructions

Nationwide Children's

Required Requisition

Send Out Instructions

Reference Test Name: Targeted Oncology Microarray
Reference Test Number: TONCMA
Instructions: Ship tumor on dry ice via FedEx Priority Overnight (Priority Plus shipping). 

Exclude from UM database.