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HelpTest Code LAB3348 Neutrophil Oxidative Burst
Clinical System Name
Neutrophil Oxidative Burst
Synonyms
Metabolic Burst Assay
Respiratory Burst Assay
Neutrophil Oxidative Burst
Neut Ox Bu
Ox Bu
OXBU
Sample Requirements
Specimen: Whole Blood
Container(s): Dark Green/Sodium Heparin (no serum separator)
Preferred Vol: 1 mL
Minimum Vol: 0.5 mL; no microtainers accepted
| Collected in-house at Seattle Children's | Collected off-site (including SCH Bellevue, SCH North and SCH South) | |
|---|---|---|
| M-Th | collect any time | collect any time |
| Friday | collect any time | ok if received at SCH main lab same day, if not do not collect |
| Saturday | ok if collected before 12 noon | do not collect |
| day before a holiday | ok if collected before 12 noon | must be received at SCH main lab before 12 noon |
| Sunday | ok if collected after 12 noon (to be tested next morning) |
ok if collected after 12 noon (to be tested next morning) |
| Sunday before a holiday Monday | do not collect | do not collect |
| Holiday | ok if collected after 12 noon (to be tested next morning) | ok if collected after 12 noon (to be tested next morning) |
Processing Instructions
Reject due to: n/a - send to lab
Spin: N
Aliquot: N
Temp: RT
Storage location: Days: Transport specimen, (copy of community services requisition, if applicable), and labels to the Cell Markers Lab (station #181). Notify Cell Markers at x72560 right away if specimen is near 20 hour specimen stability limit - billing/ordering problems should not delay delivery to lab. Eves/Nights: Store specimen, (copy of community services requisition, if applicable), and labels in the Cell Markers RT box in CPA.
Off-site collection: A normal control should accompany the specimen. It is critical that samples be kept at room temperature; use extra packing to maintain temperature. If specimen will not arrive at Children's before noon for same day testing, blood should be drawn as late in the day as possible, maintained at room temperature and sent overnight. It is recommended that samples arrive in the lab within 20-24 hours of draw, however samples may be stable for up to 50 hours. Transport specimens to Seattle Children's Hospital address on requisition: Laboratory FB.2.441, 4800 Sand Point Way NE, Seattle, WA 98105.
Stability
| Temperature | Time |
|---|---|
| Room temp | 20 hours recommended, may be stable to 50 hours, see Processing/Off-site Instructions |
| Refrigerated | N |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N |
Monday - Friday |
24 hours |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Cell Markers
Phone Number: 206-987-2560
CPT Codes
| CPT CODES |
|---|
|
88184, 88185 |
Methodology
Method: Flow Cytometry
Analytical Volume: 0.5 mL whole blood
Limitations: None specified
Reference Range
| Normal | Stimulation Index >20 |
Description
This test is a flow cytometric assay of neutrophil function utilizing the dye dihydrorhodamine (DHR). The assay is used to aid in the diagnosis of Chronic Granulomatous Disease (CGD); x-linked carrier states can also be distinguished. Note: The Nitroblue Tetrazolium Reduction (NBT) test is no longer available.