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Test Code LAB3350 NK Functional Analysis

Important Note

Collect Monday through Wednesday 1000 - 1400 ONLY. No regional site collections.

Additional Codes

NK Funct

Clinical System Name

NK Functional Analysis


Natural Killer Function

HLH: NK Function

NK Cell Function

Natural Killer Cell Function


Natural killer (NK) cells are a subset of cytotoxic lymphocytes that have the ability to induce cell death in tumor cells or in virally infected cells. NK cells play a crucial role in the homeostasis of the immune system. They are typically characterized by the expression of CD16 and CD56 on their cell surface.

Sample Requirements

Specimen: Whole Blood

Container(s): Dark Green/Sodium Heparin

Preferred Vol: See Table Below

Minimum Vol: Call Send Outs at (206) 987-2563


Note: Samples must be drawn Monday through Wednesday, 1000 - 1400 ONLY. Samples drawn outside of these times will be rejected. If drawn outside of the times, contact the Send Outs department at ext. 7-2563 for further clarification. Send sample(s) to Main Lab immediately after collection. Specimens are stable for only 24 hours at room temperature.



ALC (K/mcL) ALC (cells/mcL) mL of Sodium Heparin blood required
>1.00 >1000 5.0
1.00 1000 5.0
0.90 900 5.5
0.80 800 6.0
0.70 700 6.5
0.60 600 7.5
0.50 500 9.0
0.40 400 11.0
0.30 300 15.0
0.20* 200* 25.0*
0.10* 100* 45.0*
<0.10* <100* 50.0*


NOTE: ** For Absolute Lymphocyte Counts (ALC) below 200 cells/mcL, consider waiting until count increases.

Processing Instructions

Reject due to: Frozen, clotted, hemolyzed or centrifuged specimens. Specimen collected in an unacceptable anticoagulant.

Spin: N

Aliquot: N

Temp: RT

Storage Location: Affix a large Epic label to the sample(s), place in the room temperature Send Outs rack, and notify the Send Outs department.


Off-site Collection: No regional site collections.


Specimen Type Temperature Time
Whole Blood Room Temp 24 h
  Refrigerated Unacceptable
  Frozen Unacceptable



STAT Performed TAT
N M - F 6 d


Performing Laboratory

Cincinnati Children’s Hospital

Diagnostic Immunology Laboratory

DIL - Rm R2328

3333 Burnet Avenue

Cincinnati, OH 45229-3039


Phone: (513) 636-4685


Department: Send Outs

Phone: (206) 987-2563

CPT Codes

86352, 86357


Method: Chromium Release

Analytical Volume: See "Sample Requirements" section for more information.


Reference Range

Interpretive report is provided.

Send Out Instructions

Reference Test Name: Natural Killer Function (NK Function)
Reference Lab Test Code: 2900800
Instructions: Ship ambient, Monday through Wednesday, via FedEx Priority Overnight. Saturday deliveries are not accepted.