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Test Code LAB3355 NPM1 Insertion MRD Follow Up

Clinical System Name

NPM1 Insertion MRD Follow Up

Synonyms

AML

Acute Myeloid Leukemia

NPM

NPM-1

NPM1

NPM1 Exon 12 Insertion Mutation

Nucleophosmin

Description

This quantitative test detects common insertion mutations in NPM1 gene and is intended for post-treatment minimal residual disease monitoring in NPM1-mutated AML. Purified genomic DNA is PCR amplified using double- indexed primers coupled with sequencing adaptors for subsequent next generation sequencing using an Illumina Miseq platform.

Reportable Range: 0.007 – 50.00%
Limit of Detection: 0.003%
Limit of Quantification: 0.007%
Precision near LOD (CV): 54%

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA

Preferred Vol: 6.0 mL

Minimum Vol: 1.0 mL

 

Specimen: Bone Marrow

Container(s): Lavender/EDTA

Preferred Vol: 2.0 mL

Minimum Vol: .5 mL

 

Specimen:

Tissue, Fluids or Sorted cells: Sterile container in RPMI

Formalin-fixed paraffin embedded tissue (FFPE/PET) block

Slides: 10 unstained

Pathology should be consulted before ordering testing on FFPE specimens

 

Note: Extracted DNA will be accepted only from CLIA certified laboratories.

 

UWMC Specimen Details

For more detailed info for bone marrow testing, see separate Bone Marrow Aspirate listing .

 

Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: 2 - 4 C

Storage Location: CPA 3 refrigerator, Send Outs rack.

 

Optimal results are achieved when samples are received within 48 hours of collection for bone marrow and peripheral blood

Stability

Specimen Type Temperature Time
Whole Blood or Bone Marrow Room Temp  
  Refrigerated 4 d
  Frozen Unacceptable

 

Availability

STAT Performed TAT
N Weekly 10 d

 

Performing Laboratory

University of Washington Medical Center

Molecular Hematopathology Laboratory

825 Eastlake Avenue E, G7-800

Seattle, WA 98109

 

Phone Number: (206) 606-7060

Department

Department: Send Outs/Genetic

Phone Number: (206) 987-2563

Methodology

Method: PCR Amplification then Next Generation Sequencing

Send Out Instructions

Reference Test Name:

NPM1 Insertion Mutation by NGS
Reference Test Code: NPM1NG
Instructions: Send Monday through Thursday with the UW courier.