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Test Code LAB3365 Pancreatitis Panel

Additional Codes

Panc Pnl

Clinical System Name

Pancreatitis Panel



Hereditary pancreatitis


This next generation sequencing panel includes sequencing and deletion/duplication analysis for 5 genes: CASR, CFTR, CTRC, PRSS1 and SPINK1.

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender Top/EDTA, Yellow/ACD

Preferred Vol: 5.0 mL

Minimum Vol: 3.0 mL (1.0 mL for small infants)


Specimen: DNA

Container(s): Sterile Plastic Tube

Preferred Vol: 10 µg of purified DNA at a concentration of at least 20 µg/ml

Minimum Vol: 5 µg of purified DNA at a concentration of at least 20 µg/ml


Alternative Specimen (e.g. salvia or buccal): Alternate Specimen Collection Kits for Genetic Testing


Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temp: 2 - 8 C

Storage Location: Deliver blood to CPA refrigerator, Send Outs rack.


Off-site Collection: Send whole blood refrigerated.


Specimen Type Temperature Time
Whole Blood Room Temp 3 d
  Refrigerated 7 d
  Frozen Unacceptable
Extracted DNA Room Temp 3 - 4 d
  Refrigerated 1 y
  Frozen Indefinitely



N 3 - 4 weeks


Performing Laboratory


3800 South Business Park Avenue

Marshfield, WI 54449


Phone Number: (715) 387-0484


Department: Send Outs

Phone Number: (206) 987-2563


Reference Range

Interpretive report is provided.


For this Next Generation Sequencing (NGS) test, sequencing is accomplished by capturing specific regions with an optimized solution-based hybridization kit, followed by massively parallel sequencing of the captured DNA fragments. Additional Sanger sequencing is performed for regions not captured or with insufficient number of sequence reads. All reported pathogenic, likely pathogenic, and variants of uncertain significance are confirmed by Sanger sequencing.


For Sanger sequencing, polymerase chain reaction (PCR) is used to amplify targeted regions. After purification of the PCR products, cycle sequencing is carried out using the ABI Big Dye Terminator v.3.0 kit. PCR products are resolved by electrophoresis on an ABI 3730xl capillary sequencer. In nearly all cases, cycle sequencing is performed separately in both the forward and reverse directions.


Copy number variants (CNVs) are also detected from NGS data. We utilize a CNV calling algorithm that compares mean read depth and distribution for each target in the test sample against multiple matched controls. Neighboring target read depth and distribution and zygosity of any variants within each target region are used to reinforce CNV calls. All CNVs are confirmed using another technology such as aCGH, MLPA, or PCR before they are reported.

Special Instructions

Links to:




Clinical Utility

Prevention Genetics

Send Out Instructions

Reference Test Name: Chronic Pancreatitis Panel
Reference Lab Test Code: 10191
Instructions: Ship Monday through Friday via FedEx Priority Overnight. Saturday deliveries are accepted.