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HelpTest Code LAB3382 Plasma Hemoglobin
Clinical System Name
Plasma Hemoglobin
Synonyms
PLHB
Plasma free hemoglobin
PLASMA HB
LAB3382
Sample Requirements
Specimen: Whole blood
Container(s): Lavender/EDTA
Preferred Vol: 1.0 mL
Minimum Vol: 1.0 mL
Note: Avoid traumatic hemolysis (caused by small bore needle, vacuum suction of vacutainer, or rough handling). Plasma needs to be separated from cells within 60 minutes of collection.
Processing Instructions
Reject due to: Age of Specimen. Whole blood must be centrifuged and aliquoted within 60 mins of collection. Sample will be rejected if not removed from cells within 1 hour of collection.
Spin: N
Aliquot: N
Storage location: Core Chemistry refrigerator
Off-site collection: Specimen should be centrifuged within 1 hour of collection. Spin blood and transfer EDTA plasma to plastic tube and freeze. Send frozen.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| EDTA Plasma |
RT |
≤ 24 h |
| EDTA Plasma |
2-8 C |
≤ 24 h |
| EDTA Plasma |
-20 C or -70 C |
≤ 9 m |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y | 24/7 | 1 h |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Core Chemistry
Phone Number: (206) 987- 2617 (Client Services)
CPT Codes
83051
Methodology
Method: Spectrophotometry method by Vitros 4600
Analytical Volume: 0.25 mL
Reference Range
0 - 21 mg/dL
Description
Plasma hemoglobin is used for evaluation of hemolytic anemia. Patients on mechanical devices such as the Extra Corporal Membrane Oxygenation Device (ECMO), renal dialysis, or heart bypass pump are at risk for hemolysis.