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HelpTest Code LAB3389 Platelet Receptor Workup by Flow Cytometry
Clinical System Name
Platelet Receptor Workup by Flow
Synonyms
Platelet Glycoprotein Expression (PGE)
Bernard-Soulier Platelet Characterization by Flow Cytometry
Glanzmann Thrombasthenia Platelet Characterization by Flow Cytometry
Plt Recpt
Platelet Receptor Workup by Flow
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender/EDTA; no microtainers accepted
Preferred Vol: 2 mL
Minimum Vol: 0.5 mL
Note: Specimens stable for only 30 hours at room temperature.
| Collected in-house at Seattle Children's | Collected off-site (including SCH Bellevue, SCH North, and SCH South) | |
|---|---|---|
| M-Th | collect any time | collect any time |
| Friday | must be received before 12 noon Friday | must be received at SCH main lab before 12 noon Friday |
| Saturday | do not collect | do not collect |
| day before a holiday | ok if collected before 12 noon | must be received at SCH main lab before 12 noon day before holiday |
| Sunday | ok if collected after 12 noon (to be tested next morning) |
ok if collected after 12 noon (to be tested next morning) |
| Sunday before a holiday Monday | do not collect | do not collect |
| Holiday | ok if collected after 12 noon (to be tested next morning) | ok if collected after 12 noon (to be tested next morning) |
Processing Instructions
Reject due to: n/a - send to lab
Spin: N - DO NOT SPIN
Aliquot: N
Temp: RT
Storage location: Days: M - F until 1630, transport specimen and labels to the Cell Markers Lab (station #181). Call before tubing specimen to make sure there is someone available to do testing.
After hours: Store sample in Cell Markers RT box in CPA.
Off-site collection: Keep specimen at room temperature and transport to Children's Lab. Please contact Laboratory Client Services at (206) 987-2617 with method of transport and shipping number.
Stability
| Temperature | Time |
|---|---|
| Room temp | 30 hrs in EDTA |
| Refrigerated | N |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N |
Monday - Friday 0800-1630 |
2-3 days |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Cell Markers
Phone Number: 206-987-2560
CPT Codes
| CPT CODE(S) | |
|---|---|
| CM AB IF MALIGNANCY 1st Ab | 88184 |
| CM AB IF MALIGNANCY Add Ab | 88185 x 2 |
| CM PROFEE, Malignancy, 2-8 Markers | 88187 |
For additional information, please contact Laboratory Client Services at (206)-987-2617
Methodology
Method: Flow Cytometry
Analytical Volume: 100 uL
Limitations: None specified
Reference Range
Interpretive report will be provided.
Description
Bernard-Soulier Syndrome and Glanzmann Thrombasthenia are rare autosomal recessive platelet disorders demonstrating aberrant expression of Gp1b and GpIIb/IIIa, respectively. They can be characterized by evaluating platelet receptors by flow cytometry using monoclonal antibodies to CD41, CD42a and CD61.