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Test Code LAB339 Sickle Cell Screen

Clinical System Name

Sickle Cell Screen

Synonyms

Sickle Scrn

LAB339

Sample Requirements

Specimen: Whole Blood

Container(s):  Lavender/EDTA

Preferred Vol:  3.0 mL

Minimum Vol:  two 0.5 mL Microtainers; 1.0 mL Macrotainer

 

Notes: A Lavender top/EDTA Macrotainer must contain at least 1.0 mL.

Processing Instructions

Reject due to:  Insufficient Quantity, Wrong Sample Collection, Clotted Sample, Age of specimen

Spin: N

Aliquot: N

Temp: RT

Storage location:  Core 5 Fridge

 

Off-site collection: Store whole blood at 2-8oC and transport to Children's Lab immediately.  Samples viable for 2 weeks when stored at 2-8oC.

Stability

Specimen Type Temperature Time
Whole Blood RT

≤ 24 h

Whole Blood 2-8 C

≤ 2 w

  -20 C or -70 C

N

Availability

STAT Performed TAT
Y 24/7 1 h

Performing Laboratory

Seattle Children's Laboratory    

Department

Department:  Core Hematology

Phone Number: 206-987-2617 (Client Services)

CPT Codes

85660

Methodology

Method:   Pacific Hemostasis® SickleScreen® Sickling Hemoglobin Screening Kit 

Analytical Volume:  0.3 mL whole blood

Reference Range

Negative

 

Description

The Sickle Cell Screen is a qualitative screening test for the detection of hemoglobin S in blood samples. The test is used for the diagnosis of Sickle cell disease in conjunction with other criteria.  A hematocrit is run concurrently. Hemoglobin electrophoresis is automatically ordered to confirm positive sickle results.

 

False negatives may occur due to fetal hemoglobin; this test should not be performed on children under the age of 6 months. Suggest hemoglobin electrophoresis instead. This test is not for patients known to have Sickle cell disease.