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HelpTest Code LAB3395 Paroxysmal Nocturnal Hemoglobinuria Workup
Clinical System Name
PNH Workup
Synonyms
CD 59
CD59
FLAER
PNH Screen
PNH Workup
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender/EDTA
Preferred Vol: 4 mL
Minimum Vol: 3 mL
| Collected in-house at Seattle Children's | Collected off-site (including SCH Bellevue, SCH North and SCH South) | |
|---|---|---|
| M-Th | collect any time | collect any time |
| Friday | collect any time | ok if received at SCH main lab same day, if not do not collect |
| Saturday | ok if collected before 12 noon | do not collect |
| day before a holiday | ok if collected before 12 noon | must be received at SCH main lab before 12 noon |
| Sunday | ok if collected after 12 noon (to be tested next morning) |
ok if collected after 12 noon (to be tested next morning) |
| Sunday before a holiday Monday | do not collect | do not collect |
| Holiday | ok if collected after 12 noon (to be tested next morning) | ok if collected after 12 noon (to be tested next morning) |
Processing Instructions
Reject due to: n/a - send to lab
Spin: NO
Aliquot: NO
Temp: RT
Storage location: Days: Transport specimen and labels to the Cell Markers Lab (station #181). Eves/Nights: Store specimen and labels in the Cell Markers RT box in CPA.
Off-site collection: Keep whole blood at room temperature.
Stability
| Temperature | Time |
|---|---|
| Room temp | 24 hours |
| Refrigerated |
N |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | performed M-F, Sat before noon | 2-3 days |
Note: limited specimen stability, see sample requirements
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Cell Markers
Phone Number: 206-987-2560
CPT Codes
contact Laboratory Client Services at (206) 987-2617
Methodology
Method: Flow Cytometry
Analytical Volume: dependent on white count
Limitations:
Reference Range
Reported with results.
Description
This test is used in the workup for paroxysmal nocturnal hemoglobinuria (PNH), a rare hematopoietic stem cell disorder characterized by a somatic mutation in the PIGA gene, leading to a partial or absolute deficiency of proteins linked to the cell membrane via glycophosphatidylinositol (GPI) anchors.