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HelpTest Code LAB3395 Paroxysmal Nocturnal Hemoglobinuria Workup
Clinical System Name
PNH Workup
Synonyms
CD 59
CD59
FLAER
PNH Screen
PNH Workup
Description
This test is used in the workup for paroxysmal nocturnal hemoglobinuria (PNH), a rare hematopoietic stem cell disorder characterized by a somatic mutation in the PIGA gene, leading to a partial or absolute deficiency of proteins linked to the cell membrane via glycophosphatidylinositol (GPI) anchors.
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender/EDTA
Preferred Vol: 4 mL
Minimum Vol: 3 mL
| Collected in-house at Seattle Children's | Collected off-site (including SCH Bellevue, SCH North and SCH South) | |
|---|---|---|
| M-Th | collect any time | collect any time |
| Friday | collect any time | ok if received at SCH main lab same day, if not do not collect |
| Saturday | ok if collected before 12 noon | do not collect |
| day before a holiday | ok if collected before 12 noon | must be received at SCH main lab before 12 noon |
| Sunday | ok if collected after 12 noon (to be tested next morning) |
ok if collected after 12 noon (to be tested next morning) |
| Sunday before a holiday Monday | do not collect | do not collect |
| Holiday | ok if collected after 12 noon (to be tested next morning) | ok if collected after 12 noon (to be tested next morning) |
Processing Instructions
Reject due to: n/a - send to lab
Spin: NO
Aliquot: NO
Temp: RT
Storage location: Days: Transport specimen and labels to the Cell Markers Lab (station #181). Eves/Nights: Store specimen and labels in the Cell Markers RT box in CPA.
Off-site collection: Keep whole blood at room temperature.
Stability
| Temperature | Time |
|---|---|
| Room temp | 24 hours |
| Refrigerated |
N |
| Frozen | N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | performed M-F, Sat before noon | 2-3 days |
Note: limited specimen stability, see sample requirements
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Cell Markers
Phone Number: 206-987-2560
Methodology
Method: Flow Cytometry
Analytical Volume: dependent on white count
Limitations:
Reference Range
Reported with results.
CPT Codes
contact Laboratory Client Services at (206) 987-2617