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HelpTest Code LAB3414 Protoporphyrins, Fractionation, Whole Blood
Reporting Name
Protoporphyrins, Fractionation, WBUseful For
Evaluating patients with possible diagnoses of erythropoietic protoporphyria or X-linked dominant protoporphyria
Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria
Testing Algorithm
This test should not be ordered in conjunction with PEE / Porphyrins Evaluation, Whole Blood.
The following information is available:
-Porphyria (Acute) Testing Algorithm
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole bloodOrdering Guidance
This test is for assessment for protoporphyria. The preferred test for lead toxicity in children is blood lead. For more information see PBDV / Lead, Venous with Demographics, Blood or PBDC / Lead, Capillary, with Demographics, Blood. The preferred screening test for suspicion of a hepatic porphyria is urine porphyrins. For more information see PQNRU / Porphyrins, Quantitative, Random, Urine. This test should not be ordered with PEE / Porphyrins Evaluation, Whole Blood
Necessary Information
Include a list of medications the patient is currently taking.
Specimen Required
All porphyrin tests on whole blood can be performed on 1 tube.
Patient Preparation: Patient must not consume any alcohol for 24 hours before specimen collection.
Container/Tube:
Preferred: Green top (sodium heparin)
Acceptable: Dark blue top (metal free heparin), green top (lithium heparin), or lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions: Refrigerate specimen as soon as possible.
Specimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole blood | Refrigerated | 7 days |
Special Instructions
Reference Values
FREE PROTOPORPHYRIN
<20 mcg/dL
ZINC-COMPLEXED PROTOPORPHYRIN
<60 mcg/dL
Day(s) Performed
Monday through Friday
TAT: 4 - 5 days
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82542
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PPFE | Protoporphyrins, Fractionation, WB | 94490-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 2327 | Zinc-Complexed Protoporphyrin | 2895-1 |
| 2326 | Free Protoporphyrin | 94491-8 |
| 29511 | Interpretation | 59462-2 |
Method Description
Extraction followed by fractionation by high-performance liquid chromatography. Zinc protoporphyrin and free protoporphyrin are separately quantitated.(Smith RM, Doran D, Mazur M, Bush B. High-performance liquid chromatographic determination of protoporphyrin and zinc protoporphyrin in blood. J Chromatogr.1980;181[3-4]:319-327; Gou EE, Balwani M, Bissell DM, et al. Pitfalls in erythrocyte protoporphyrin measurement for diagnosis and monitoring of protoporphyrias. Clin Chem. 2015;61[12]:1453-1456. doi:10.1373/clinchem.2015.245456)
Report Available
3 to 5 daysReject Due To
| Gross hemolysis | Reject |
Method Name
High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection
Forms
If not ordering electronically, complete, print, and send a Biochemical Genetics Test Request (T798) with the specimen.