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Test Code LAB3449 SCID Newborn Screen Follow-up Panel by Flow Cytometry

Important Note

For Newborn Screen Follow-up ONLY

Note: limited specimen stability - see Sample Requirements and Processing Info below - do NOT refrigerate.

For more information, call Cell Markers Lab at (206) 987-2560.

Offsite collection:  If specimen will not arrive at Children's before noon for same day testing, blood should be drawn as late in the day as possible, maintained at room temperature and sent overnight with first morning delivery to allow test set-up the following morning within the specimen stability window. See additional notes under 'Processing Instructions - Offsite Collection'.

Clinical System Name

SCID Newborn Screen Follow-up Panel

Description

Immunology followup for positive SCID Newborn Screen ONLY - includes CD2, CD3, CD4, CD8, CD16, CD19, CD56, HLA-DR, CD4+CD45RA/RO and CD8+CD45RA/RO.

Sample Requirements

Specimen:  Whole Blood

Container(s):  Lavender/EDTA; Dk Green/Na Heparin also acceptable from referring labs if CBC & differential results submitted from same collection

Preferred Vol:  2 - 3 mL

Minimum Vol:  2 mL; no microtainers

Note:  Specimens received after 1430 will be held and testing will be performed the next working day. 

  Collected in-house at Seattle Children's Collected off-site (including SCH Bellevue, SCH North and SCH South)
M - Th collect any time collect any time
Friday collect any time ok if received at SCH main lab same day, if not do not collect
Saturday ok if collected before 12 noon do not collect
day before a holiday ok if collected before 12 noon must be received at SCH main lab before 12 noon
Sunday ok if collected after 12 noon (to be tested next morning) ok if collected after 12 noon (to be tested next morning)
Sunday before a holiday Monday do not collect do not collect
Holiday ok if collected after 12 noon (to be tested next morning) ok if collected after 12 noon (to be tested next morning)

Processing Instructions

Reject due to:  clotted specimen, microtainer, refrigerated

Spin: N

Aliquot: N

Temp:  RT - DO NOT REFRIGERATE

Note:  Request Core Lab perform a CBC with differential and ensure a copy of the results is sent with the specimen to Cell Markers. Shared specimens with CBC/Diff orders do not need to repeat testing, but a copy of the results should be printed to accompany the specimen to the Cell Markers Lab. Lavender top Community Service specimens with external CBC Diff results should still be processed by Core for an in-house CBC Diff for comparison if within stability.

Storage location: Days: Transport specimen, Core Lab results, and copy of community services requisition (if applicable), and labels to the Cell Markers Lab (station #181). Eves/Nights: Store specimen, Core Lab results, and copy of community services requisition (if applicable), and labels in the Cell Markers RT box in CPA.

Off-site collection:  Keep whole blood at room temperature. If specimen will be greater than 24 hours old when received at Seattle Childrens, referring labs must send CBC w/differential results from the same collection along with specimen.  EDTA can be tested up to 30 hours, Na Heparin can be tested up to 48 hours.

Stability

Temperature Time
Room temp EDTA - 30 hours; Heparin - 48 hours
Refrigerated N
Frozen N

Performing Laboratory

Seattle Children's Laboratory

Department

Department:  Cell Markers

Phone Number: 206-987-2560

 

Synonyms

SCID Screen

SCID NB F/U

SCID Newborn Screen

SCID Newborn Screen Follow-up Panel

Availability

STAT Performed TAT
N M-F, Sa (0730-1200) 2-3 days

Note: limited specimen stability - see Sample Requirements and Processing Info.

Methodology

Method:  Flow Cytometry

Analytical Volume:  dependent on patient's white count and testing requested

Limitations: refrigeration selectively decreases subsets - refrigerated samples not acceptable.

Reference Range

Reference values accompany patient report.

CPT Codes

contact Laboratory Client Services at (206)-987-2617

Requisition

NBS SCID Requisition