Sign in →

Test Code LAB3458 Soluble C5b-9 Assay

Clinical System Name

Soluble C5b-9 Assay

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA

Preferred Vol: 1.5 mL

Minimum Vol: 1.0 mL

Note: Deliver to lab immediately.

Processing Instructions

Reject due to: Incorrect anticoagulant. Hemolyzed, clotted, or older than 2 hours unspun.

Spin: Y

Aliquot: Y

Temp: -20 C

Storage Location: Separate plasma from cells within TWO hours of collection. Spin bood and transfer 0.5 mL plasma (Min. 0.2 mL) to an aliquot tube affixed with large Epic label. Place sample in the CPA freezer Fz3 rack.

 

Off-site Collection: Separate plasma from cells within TWO hours of collection. Spin bood and transfer 0.5 mL plasma (Min. 0.2 mL) to an aliquot tube affixed with large Epic label. Freeze at -20 C. Transport frozen.

Stability

Specimen Type Temperature Time
Whole Blood RT 2 h
  2-8 C 2 h
Plasma RT 8 h
  2-8 C 24 h
  -20 C 6 m

 

Performing Laboratory

Seattle Children's Laboratory

Department

Department: Chemistry

Phone Number: (206) 987-2617

Synonyms

MAC (SC5b-9)

Eculizumab Monitor (SC5b-9)

Membrane Attack Complex (SC5b-9)

Soluble Terminal Complement Complex (SC5b-9)

Availability

Samples in lab by 0900 Monday will be resulted by 1800 Monday. 

Contact Chemistry Department (206-987-2565, LabChemistry@seattlechildrens.org) by 1500 Wednesday for approval to set-up an additional Thursday run.

STAT Performed TAT
N M 7 d

Note: If Monday is a holiday, then the run may be set-up on Monday or Wednesday.

 

 

Methodology

Method: EIA on the DS2

Analytical Volume: 0.2 mL Plasma

Limitations:

Reference Range

< 250 ng/mL

CPT Codes

86160