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HelpTest Code LAB348 Urinalysis with Microscopic
Clinical System Name
Microscopic with Urinalysis
Synonyms
UA/MICRO
LAB348
Sample Requirements
Specimen: Urine
Container(s): Sterile, screw-capped container preferred. Clean container also acceptable.
Preferred Vol: 12.0 mL fresh random urine
Minimum Vol: 2.0 mL
Notes: Specimen must be received in the Lab within two hours. If there will be a delay, keep the specimen refrigerated (up to 10 hours) and transport on ice. Do not use cotton balls or gauze to collect specimen. Do not squeeze urine from diapers.
For bagged urines: Aspirate urine sample from bag using a sterile needleless syringe and place in sterile specimen cup before sending to the lab.
For non-sterile containers: Urines collected in non-sterile containers will not be sent for culture. They will have the following comment appended: “Urine collected into a non-sterile container. Results may be affected due to unknown interferents, and sample is unsuitable for culture. For future collections please collect urine into a sterile specimen cup.”
UA testing cannot be performed off 24 hour urine collections.
Acceptable collection methods: bagged, bladder tap, bladder wash, clean catch, cystoscopy, direct catheter, foley catheter, lleal loop, indwelling catheter, kidney, midstream void, nephrostomy, suprapubic aspirate, ureter. The urinalysis includes the biochemical tests listed in the reference range and specific gravity.
Processing Instructions
Reject due to: Insufficient quantity, incorrect storage temperature, age of specimen, gross contamination, specimens collected with cotton balls or gauze, specimens obtained from diapers, specimens sent in any preservative solution.
Spin: N
Aliquot: N
Temp: RT
Storage location: Core 5 Fridge
Off-site collection: Deliver specimen to the Lab within two hours of collection or keep refrigerated at 2-8oC.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | RT |
≤ 2 h |
| Urine | 2-8 C |
≤ 10 h |
| -20 C or -70 C |
N |
Availability
| STAT | Performed | TAT |
|---|---|---|
| Y | 24/7 | 1 h |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Core Urinalysis
Phone Number: 206-987-2617 (Client Services)
CPT Codes
81001
Methodology
Method: Sysmex UN-3000 and Clinitek Advantus Urine Analyzer with Seimens Reagent strips: Chemistry analyzer, urine particle analytical module, urine sediment digital imager.
Analytical Volume: 3.5 mL
Reference Range
|
Biochemical tests |
Reference Values |
|
Leukocyte Esterase |
Negative |
|
Specific Gravity (Random Specimen) |
1.001 - 1.035 |
|
pH |
4.5 - 8.0 |
|
Qualitative Protein |
Negative |
|
Glucose |
Negative |
|
Ketones (Acetone) |
Negative |
|
Bilirubin |
Negative |
|
Blood (Hemoglobin) |
Negative |
|
Nitrites |
Negative |
|
Urobilinogen |
Less than or equal to 1.0 mg/dL. |
|
|
|
|
Microscopic Results |
|
|
RBCs |
Up to 5/hpf. |
|
WBCs |
Up to 5/hpf. |
|
Crystals |
Most are diet dependent. |
|
Casts - hyaline |
May be present, relative to clinical picture. |
|
Casts - non-hyaline |
None seen. |
|
Bacteria |
None to few. Presence is relative to specimen collection. |
|
Epithelial Cells |
None to few. More than a few can indicate non-clean collection. |
Result Reporting Effective Dec. 10, 2024
|
|
Current Reporting |
New Reporting |
|
Urine Color |
Yellow, Straw, Slt. Blood, Bloody, Amber, Bright Yellow, Orange, Green, Brown, Colorless, Red, Other |
Yellow, Dark Yellow, Orange, Red, Green, Other |
|
Urine pH |
≤ 5.0, 5.5, 6.5, 7.0, 7.5, 8.0, 9.0, ≥ 9.0 |
≤ 5.0, 5.5, 6.5, 7.0, 7.5, 8.0, 9.0, ≥ 9.0 |
|
Urine Leukocyte Esterase |
Negative, Trace, 1+, 2+, 3+ |
Negative, Trace, 1+, 2+, 3+ |
|
Urine Nitrite |
Negative, Positive |
Negative, Positive |
|
Urine Protein (Qualitative) |
Negative, Trace, 1+, 2+, 3+ |
Negative, Trace, 1+, 2+, 3+, 4+ |
|
Urine Glucose (Qualitative) |
Negative, Trace, 1+, 2+, 3+ |
Negative, Trace, 1+, 2+, 3+ |
|
Urine Ketones |
Negative, Trace, 1+, 2+, 3+ |
Negative, Trace, 1+, 2+, 3+, 4+ |
|
Urine Urobilinogen |
≤ 0.2, 1.0, 2.0, 4.0, ≥ 8.0 |
≤ 0.2, 1.0, 2.0, 4.0, ≥ 8.0 |
|
Urine Bilirubin |
Negative, 1+, 2+, 3+, Positive |
Negative, 1+, 2+, 3+, Positive |
|
Urine Occult Blood |
Negative, Trace, 1+, 2+, 3+, Positive |
Negative, Trace, 1+, 2+, 3+, Positive |
|
Urine RBCs |
None, 0-2, 3-10, 11-20, 21-100, >100 |
0-2, 3-5, 6-10, 11-20, 21-50, 51-100, >100 |
|
Urine WBCs |
None, 0-4, 5-10, 11-20, 21-100, >100 |
None, 0-5, 6-10, 11-20, 21-50, 51-100, >100 |
|
Urine Epithelial Cells, Transitional Epithelial Cells |
None, Rare, Few, Moderate, Many |
0-2, 3-5, 6-10, 11-20, >20 |
|
Urine Bacteria |
None Seen, Rare, Few, Moderate, Many |
None Seen, Occ., Few, Moderate, Many |
|
Hyaline Casts, Granular Casts, Renal Tubular Cells |
Rare, 1-5, 6-10, 11-20, 21-100, >100 |
0-2, 3-5, 6-10, 11-20, >20 |
|
WBC Clumps |
Rare, Few, Moderate, Many |
Rare, Few, Moderate, Many |
|
Yeast, Urine Oval Fat bodies, Urine Fat Free Drops, Trichomonas |
Rare, Few, Many |
Present (if seen) |
|
Urine Dysmorphic RBCs, Sperm |
Present (if seen) |
Present (if seen) |
|
Urine Amorphous Crystals, Calcium Oxalate Crystals, Uric Acid Crystals, Triple Phosphate Crystals, Ammonium Biurate Crystals, Bilirubin Crystals, Calcium Carbonate Crystals, Calcium Phosphate Crystals, Calcium Sulphate Crystals, Cystine Crystals, Unidentified Urine Crystals |
Few, Many |
Present (if seen) |
|
WBC Casts, RBC Casts |
None, Rare, 1-5, 6-10, 11-20, 21-100, >100 |
None, Rare, 1-5, 6-10, 11-20, 21-100, >100 |
|
Waxy Casts, Fatty Casts, Broad Casts, Heme GR Casts |
None, 0-1, 1-5, 6-10, 11-20, 21-100, >100 |
None, 0-1, 1-5, 6-10, 11-20, 21-100, >100 |