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Test Code LAB3490 Testosterone Total by LC/MS

Clinical System Name

Testosterone Total by LC/MS

Synonyms

Total Testosterone
TESTOST MS

Sample Requirements

Specimen: Whole Blood

Container(s): Red, Gold/SST, Dark Green/Sodium Heparin, or Lt. Green/Lithium Heparin

Preferred Vol: 2.0 mL

Minimum Vol: 1.0 mL

 

Note: Lavender/EDTA samples are NOT acceptable for this testing. Send the sample(s) to the Main Lab ASAP. Serum or plasma must be separated from the cells within TWO hours of collection.

Processing Instructions

Reject due to: EDTA plasma

Spin: Y

Aliquot: Y

Temp: 2 - 8 C

Storage Location: Transfer 200 mcL serum to plastic aliquot container affixed with large Epic label. Freeze in CPA Freezer, Fz6 rack. Store label with specimen.

 

Off-site Collection: Separate serum or plasma from cells ASAP or within TWO hours of collection. Transfer 1.0 mL serum or plasma to a plastic aliquot tube (Min: 0.2 mL).

Stability

Specimen Type Temperature Time
Serum or Plasma Room temp 24 h
  Refrigerated

1 m
  Frozen 6 m

 

Availability

STAT Performed TAT
N T, Th 2 - 6 d

 

Performing Laboratory

Seattle Children's Laboratory

 

Department

Department:  Chemistry

Location: Chem West

Phone Number: (206) 987-2617

CPT Codes

84403

Methodology

Method: Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Analytical Volume: 0.2 mL Serum or Plasma

Limitations:

Reference Ranges

Age Male (ng/dL)
Newborn 75 - 400
1 - 5 months 14 - 400
6 - 24 months Less than 37
2 - 7 years Less than 19
8 - 9 years Less than 8
10 - 11 years Less than 165
12 - 15 years Less than 733
16 years and older 158 - 1080
Tanner Stage I 2 - 15
Tanner Stage II 3 - 303
Tanner Stage III 10 - 851
Tanner Stage IV - V 162 - 847

 

Age Female (ng/dl)
Newborn 20 - 64
1 month - 5 years Less than 30
6 - 9 years Less than 11
10 -11 years Less than 32
12 years and older 6 - 58
Tanner Stage I 2 -17
Tanner Stage II 5 - 40
Tanner Stage III 10 - 63
Tanner Stage IV - V 11 - 62