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Test Code LAB3551 Von Willebrand Factor Collagen Binding

Additional Codes

VWFCB

Clinical System Name

Von Willebrand Factor Collagen Binding

Sample Requirements

Specimen: Whole Blood

Container(s): Lt. Blue/Citrate

Preferred Vol: (1) 2.7 mL

Minimum Vol: (1) 1.8 mL

 

Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions. 

Processing Instructions: Main Campus

Deliver whole blood to Coag bench. Coag Technologist will process.

 

Reject due to: clotted, hemolyzed, insufficient quantity (underfill), or improper collection (overfill).

Spin: Y

Aliquot: Y

Temp: -70 C

Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.

 

Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

Processing Instructions: Offsite & Regional Clinics

Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).

Spin: Y

Aliquot: Y

Storage location:  -70 C (preferred) or -20 C. 

 

Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool. 

 

Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.

 

Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.

Stability

Specimen Type Temperature Time
Plasma Room temp N
  Refrigerated N
  Frozen Y

 

Performing Laboratory

Harborview Medical Center

Clinical Coagulation Lab

325 9th Ave

Room GWH 47

Seattle, WA 98195

 

Phone Number: (206) 744-3128

Department

Department: Send Outs

 

Phone Number: (206) 987-2563

Send Out Instructions

Reference Test Name: Von Willebrand Factor Collagen Binding
Reference Lab Test Code: VWFCBA
Instructions: Send out Monday through Friday with the UW courier.

 

Synonyms

VWF Collagen Binding; VWFCB

Availability

STAT Performed TAT
N Weekly 7 - 10 d

 

Methodology

Method: Enzyme Linked Immunosorbent Assay (ELISA)

Analytical Volume: 0.5 mL Plasma

Limitations:

CPT Codes

85245