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HelpTest Code LAB3551 Von Willebrand Factor Collagen Binding
Additional Codes
VWFCB
Clinical System Name
Von Willebrand Factor Collagen Binding
Synonyms
VWF Collagen Binding; VWFCB
Sample Requirements
Specimen: Whole Blood
Container(s): Lt. Blue/Citrate
Preferred Vol: (1) 2.7 mL
Minimum Vol: (1) 1.8 mL
Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid for detailed instructions.
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma | Room temp | N |
| Refrigerated | N | |
| Frozen | Y |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | Weekly | 7 - 10 d |
Performing Laboratory
Harborview Medical Center
Clinical Coagulation Lab
325 9th Ave
Room GWH 47
Seattle, WA 98195
Phone Number: (206) 744-3128
Department
Department: Send Outs
Phone Number: (206) 987-2563
Methodology
Method: Enzyme Linked Immunosorbent Assay (ELISA)
Analytical Volume: 0.5 mL Plasma
Limitations:
CPT Codes
85245
Processing Instructions: Main Campus
Deliver whole blood to Coag bench. Coag Technologist will process.
Reject due to: clotted, hemolyzed, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Temp: -70 C
Storage Location: CPA 2 Freezer ( -70 C) Send Out rack.
Specimen should be centrifuged within one hour of collection. Transfer upper 3/4 layer of plasma to plastic tube affixed with large Epic aliquot label. Freeze one aliquot of plasma at -70 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Send Out Instructions
| Reference Test Name: | Von Willebrand Factor Collagen Binding |
| Reference Lab Test Code: | VWFCBA |
| Instructions: | Send out Monday through Friday with the UW courier. |
Processing Instructions: Offsite & Regional Clinics
Reject due to: clotted, insufficient quantity (underfill), or improper collection (overfill).
Spin: Y
Aliquot: Y
Storage location: -70 C (preferred) or -20 C.
Specimen should be centrifuged within one hour of collection. Double spin, transfer upper 3/4 layer of plasma to plastic tube affixed with large sample label. Freeze one aliquot of plasma at -70 C (preferred) or -20 C. Preferred plasma volume: 1.0 mL, minimum plasma volume: 0.6 mL. Do not pool.
Non-Children's Hospital Offsite collection: Ship completely frozen on dry ice.
Children's Hospital Regional Clinic collection: Ship completely frozen in frozen Nalgene Labtop cooler with ice pack inside an insulated soft cooler.