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Test Code LAB3558 Wilm's Tumor Loss of Heterozygosity

Important Note

Pre-treatment tumor samples are strongly preferred.

Required Requisition

If submitting a tumor sample collected during or after treatment (e.g. post-chemotherapy), please indicate post-treatment status of the sample on the requisition form.

Additional Codes

Wilm's Tumor LOH

Clinical System Name

Wilm's Tumor Loss of Heterozygosity

Synonyms

Wilm's Tumor LOH

Sample Requirements

Specimen: Tumor

Container(s): Sterile Screw-Capped Container

Preferred Vol: 0.05 g of snap‐frozen tumor tissue is preferred.

Alternative: OCT‐embedded or paraffin embedded tumor tissue (tissue scrolls or paraffin block) also accepted.

Note: At least 40% tumor must be present in the submitted sample (based on internal pathology review). Snap-frozen tissue is preferred.

 

Nationwide Children's Test Information

Processing Instructions

Reject due to: Delayed or improper handling; Insufficient tumor content in tumor sample; Inadequate tissue; Tissue degradation; Wrong type of specimen

Spin: N

Aliquot: N

Temp: -70 C for Tumor Biopsy

Storage Location: Deliver tumor biopsy to the CPA -70 freezer, Send Outs rack.

 

Off-site collection:

Stability

Specimen Type Temperature Time
Paraffin Block Room Temp 6 m
Tissue Scrolls (FFPE) Room Temp 6 m
Tissue (Snap-frozen) Room Temp Unacceptable
  Refrigerated Unacceptable
  Frozen 24 h

 

Availability

STAT Performed TAT
N M - F 3 w

 

Performing Laboratory

Nationwide Children's Hospital

Nationwide Children's Laboratory Services
700 Children’s Drive, Room C1955
Columbus, OH 43205

 

Laboratory Client Services
 (614) 722-5477

Department

Department: Send Outs/Genetic

Phone Number: (206) 987-2563

Methodology

Method: Microarray Analysis

This test evaluates for the following clinically significant aberrations within the cancer genome for Wilms Tumor:

  • Copy number loss of 1p, 11p15, 16q, and 17p
  • Copy number gain of 1q
  • Loss of Heterozygosity involving 1p, 11p15, 16q, and 17p

Information regarding other chromosome regions will not be reported, as their clinical significance in Wilms tumor is currently unknown.

Reference Range

Interpretive report is provided

Special Instructions

Nationwide Children's

Required Requisition

Send Out Instructions

Reference Test Name: Targeted Oncology Microarray Analysis
Reference Test Number: TONCMA
Instructions:

Ship tumor on dry ice via FedEx Priority Overnight (Priority Plus shipping). 

Exclude from UM database.