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Test Code LAB3573 Flow Cytometry at UW/FHCC

Important Note

Flow Cytometry at UW/FHCC is ONLY AVAILABLE FOR BMT AND HEM/ONC PROVIDERS.  Orders will be reviewed by hematopathologist prior to sending. If you would like flow cytometry performed at Seattle Children’s Laboratory, please contact hematopathology and DO NOT place this order.

 

Clinical System Name

Flow Cytometry at UW/FHCC

Synonyms

Flow Cytometry; Immunophenotyping

Description

This sendout order is only available for BMT and Hem/onc providers. Orders will be reviewed by hematopathologist prior to sending. If you would like flow cytometry performed at Seattle Children’s Laboratory, please contact hematopathology and do not place this order.

Sample Requirements

Specimen Type: Blood or Bone Marrow Aspirate

Container Type(s): Dark Green NaHep or Lavender Top Tube

Preferred Vol: 6.0 mL

Minimum Vol: 1.0 mL

 

Note: Deliver with other collections to Children's Laboratory. For bone marrow and peripheral blood testing, Lavender Top Tubes are acceptable, but not preferred.

 

For CSF Flow Cytometry (BMT/HSCT Patients)

Specimen Type: Cerebrospinal Fluid

Container Type(s): CSF Tube

Preferred Vol: 5.0 mL

Minimum Vol: Entire Sample

 

Specimen Type: Body Fluid (Varies)

Container Type(s): Sterile Container

Preferred Vol: 5.0 mL

Minimum Vol: Entire Sample

 

Specimen Type: Tissue

Container Type(s): Sterile Container

Preferred Vol: Entire Sample

Minimum Vol: Entire Sample

Processing Instructions

Reject due to:

Spin: N

Aliquot: N

Temperature:

  • Bone Marrow: Room Temp
  • CSF, Body Fluid, and Tissue: 2-4 C in RPMI.

Storage Location:

  • Bone Marrow: Room temperature Send Outs rack
  • CSF, Body Fluid, and Tissue: CPA1 refrigerator, Send Outs rack (after RPMI has been added)

 

Note: For CSF Send-Out: Cells in CSF are extremely fragile. RPMI should be added in approximately 1:1 ratio before sending out for additional testing. Send Outs will contact Cell Markers Lab to add RPMI (or prealiquoted RPMI also available in Histology refrigerator).

 

Off-site Collection: Contact UW Hematopathology at (206) 606-7060 for instructions.

Stability

Specimen Type Temperature Time
Bone Marrow Room Temp  
  Refrigerated Unacceptable
  Frozen Unacceptable

 

Specimen Type Temperature Time
CSF, Body Fluids, and Tissue Room Temp Unacceptable
  Refrigerated  
  Frozen Unacceptable

Availability

STAT Performed TAT
N Daily 1 - 5 days

 

Performing Laboratory

Fred Hutch Cancer Center (FHCC)

UW Hematopathology

825 Eastlake Ave E, G1500

Seattle, WA 98195

 

Phone: (206) 606-7060

Department

Department: Send Outs

Phone Number: (206) 987-2563

Methodology

Method: Flow Cytometry

Analytical Volume: See Below

  • Whole Blood: 1.0 mL
  • Bone Marrow: First pull collected in Lavender Top Tube or Dark Green Sodium Heparin
  • Tissues: Entire Sample
  • CSF and BAL: Entire Sample
  • Body Fluids: Entire Sample

Limitations: None Specified

Send Out Instructions

Reference Lab Test Name: Flow Cytometry (Surface Marker Profile)
Reference Lab Test Code: SMP
Instructions: Send to FHCC/UW Hematopathology Lab via Delivery Express courier.