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Test Code LAB3588 Human Herpes Virus 8 quant PCR blood/CSF

Additional Codes

HH8QN

Clinical System Name

Human Herpes Virus 8 quant PCR blood/CSF

Synonyms

HH8 PCR, HHV-8, Human Herpes 8 by PCR, Kaposi Sarcoma Virus, KS Virus by PCR, Viral load-HHV8

Sample Requirements

Specimen: Whole Blood or CSF

Container(s): Lavender/EDTA or CSF Tube

Preferred Vol: 3.0 mL Whole Blood or 1.0 mL CSF

Minimum Vol: 1.0 mL Whole Blood or 0.5 mL CSF

 

Note: This test can share a tube with other Viral PCR testing but a separate tube needs to be collected if Hematology (CBC, Platelet, ESR, etc) testing is ordered.

Processing Instructions

Reject due to: Dark Green/Sodium Heparin

Spin: Y

Aliquot: Y

Temp: -20 C

Storage Location: Spin blood within two hours of collection, transfer 1.0 mL of plasma to a plastic aliquot and put into Send Outs rack of the CPA -20 freezer. Aliquot and freeze CSF within two hours of collection if possible (can be refrigerated up to 48 hours).

Monday – Friday: Place sample in the Virology rack in the CPA freezer.

Saturday-Sunday & Holidays: Close batch in Mayo Access and courier to UW NW220.

 

Off-site Collection:

  • Blood: Centrifuge tube within two hours of collection, aliquot plasma, and freeze at -20°C.
  • CSF: Freeze within two hours of collection or ship immediately at 4°C. If CSF has cells, spin and aliquot clear CSF, then freeze at -20°C.

Stability

Specimen Type Temperature Time
  Room Temp

 

  Refrigerated 48 h
  Frozen

Indefinitely

 

Availability

STAT Performed TAT
N M, W, F 1 - 3 d

 

Performing Laboratory

University of Washington

Department of Laboratory Medicine

UW Molecular Virology Lab

1959 NE Pacific St, NW220
Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

87799

Methodology

Method: Real-Time Polymerase Chain Reaction (PCR)

Analytical Volume: 0.5 mL Plasma or CSF

Limitations:

Send Out Instructions

Reference Test Name: HHV8 PCR, Quantitative
Reference Test Number:

HH8QN

Instructions:

Send out daily with the UW courier. Weekends and Holidays courier on dry ice to UW NW220.